IN VITRO ASSESSMENT OF CYTOTOXICITY AND OXIDATIVE STRESS PARAMETERS OF NATURAL RUBBER LATEX MEMBRANES STABILIZED WITH DIFFERENT CONCENTRATIONS OF AMMONIA IN CHO-K1 CELLS

Authors

  • Caroline de Souza Araujo UNOESTE
  • Dalita Gomes Silva Morais Cavalcante Unesp
  • Nathália Oliveira Braga Unesp
  • Andressa Silva Gomes Unesp
  • Eidi Yoshihara APTA
  • Leandra Ernst Kerche UNOESTE
  • Aldo Eloizo Job Unesp

Keywords:

Natural rubber latex, membranes, ammonia, cytotoxicity, GSH.

Abstract

The purpose of this study was to assess in vitro cytotoxicity of natural rubber (NR) latex membranes stabilized with different concentrations of ammonia, as well as evaluate possible changes in oxidative stress parameters. To this end, CHO-k1 cells were exposed to NR membranes for 24 h. Cytotoxicity was assessed by the MTT assay and the oxidative stress parameters were established by glutathione (GSH) and malondialdehyde (MDA) levels. The results show that the ammonia present in the NR latex membranes can deplete cell viability and enhance GSH consumption in the cells. NR latex membranes stabilized with 2.00% of ammonia were cytotoxic to the cell lineage used in this study. From these results, it is possible to infer that the use of ammonia to stabilize NR latex membranes with biomedical purposes has to be done carefully, to avoid possible harmful effects to human health.

Downloads

Download data is not yet available.

Author Biography

  • Leandra Ernst Kerche, UNOESTE
    Graduada em Ciências Biológicas pela UEL (2005), Mestre em Biotecnologia pela UEL (2010) e Doutora em Biomateriais pela Unesp de Pres. Prudente, realiza pós-doutorado em Oncologia pelo AC Camargo Cancer Center. Orienta trabalhos com ênfase em Biomateriais, Mutagênese e Genética do Câncer.

Downloads

Published

2020-01-17

Issue

Vol. 11 No. 3 (2019): Colloquium Vitae, Vol. 11, n. 3

Section

Artigos Originais

License

Os artigos submetidos à revista Colloquium Vitae estão licenciados conforme CC BY-NC-ND. Para mais informações sobre essa forma de Licenciamento, consulte: http://creativecommons.org/licenses/by-nc-nd/4.0/.
A disponibilização é gratuita na Internet, para que os usuários possam ler, fazer download, copiar, distribuir, imprimir, pesquisar ou referenciar o texto integral dos documentos, processá-los para indexação, utilizá-los como dados de entrada de programas para softwares, ou usá-los para qualquer outro propósito legal, sem barreira financeira, legal ou técnica.

How to Cite

IN VITRO ASSESSMENT OF CYTOTOXICITY AND OXIDATIVE STRESS PARAMETERS OF NATURAL RUBBER LATEX MEMBRANES STABILIZED WITH DIFFERENT CONCENTRATIONS OF AMMONIA IN CHO-K1 CELLS. (2020). Colloquium Vitae. ISSN: 1984-6436, 11(3), 32-40. https://journal.unoeste.br/index.php/cv/article/view/2446

Most read articles by the same author(s)