EVALUATION OF DIFFERENT FUNGO PRESERVATION METHODS Phoma dimorpha
PRESERVATION Phoma dimorpha
Keywords:
crescimento micelial, repicagens periódicas, Castellani, fragmentos de papel-filtroAbstract
Facing the need for preservation and maintenance of biological materials, among them fungi with potential for biological control, for biotechnological and scientific development, which are gaining prominence in the world scenario. It is necessary to adapt preservation methods that besides guaranteeing the survival of these microorganisms allow the conservation of their morphological, physiological and genetic characteristics, however, there is no ideal or universal method for the conservation of biological materials. In view of this need, the present work had the objective of evaluating the efficacy and feasibility of three methods for the preservation of Phoma dimorpha (periodic repictions, Castellani and filter paper fragments) isolates in two evaluation periods, six and twelve months after the storage. Efficacy and viability were studied by mycelial growth of the fungus in a culture medium containing potato-dextrose-agar. There was variability between the preservation methods of the P. dimorpha fungus isolate for mycelial growth, method efficacy and mycelial growth rate index, in the two evaluation periods, after six and twelve months of storage. The filter paper fragment preservation method was the most effective in preserving the P. dimorpha fungus isolate in the two evaluation periods, after six and twelve months of storage, being ideal to obtain the highest mycelial growth, efficacy of the method and mycelial growth rate index.
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References
ABBOTT, W. S. A method of computing the effectiveness of an insecticide. Journal of Economic Entomology, v.18, n.2, p. 265-267, 1925. https://doi.org/10.1093/jee/18.2.265a
ALFENAS, A. C.; MAFIA, R. G. Métodos em fitopatologia. 2. ed. Viçosa: UFV, 2007. 516 p.
ANJOS, T. V.; TEBALDI, N. D.; FAGIAN, C. C. Coleção preservação de culturas de bactérias fitopatogênicas. Horizonte Científico, v.6, n.1, p. 1-24, 2012.
APARECIDO, C. C.; HUANG; C. T. M.; PASSADOR, M. M.; FINATTI, D.; FIGUEIREDO, M. B. Divulgação técnica: avaliação da viabilidade de culturas fúngicas preservadas pelos métodos de Castellani (água destilada) e liofilização. Biológico, v.69, n.1, p. 5-8, 2007.
APARECIDO, C. C.; PIRES, G. C. C.; FINATTI, D.; CAMILO, C. M. Preservação de micro-organismos a -80º C. Biológico, v.74, n.1, p. 23-29, 2012.
ARORA, P.; WANI, Z. A.; NALLI, Y.; ALI, A.; RIYAZ-UL-HASSAN, S. Antimicrobial potential of Thiodiketopiperazine derivatives produced by Phoma sp., an endophyte of Glycyrrhiza Glabra Linn. Microbial Ecology, v.72, n.4, p. 802-812, 2016. http://dx.doi.org/10.1007/s00248-016-0805-x
BELOTI, I. F.; JULIATTI, B. C. M.; JULIATTI, F. C. Evaluation of the gelatin technique for the preservation of phytopathogenic fungi. Bioscience Journal, v.33, n.4, p. 923-932, 2017. http://dx.doi.org/10.14393/BJ-v33n4a2017-37116
BRUN, T.; RABUSKE J. E.; TODERO, I.; ALMEIDA, T. C.; JUNIOR J. J. D.; ARIOTTI, G.; CONFORTIN, T.; ARNEMANN, J. A.; KUHN, R. C.; GUEDES, J. V. C.; MAZUTTI, M. A. Production of bioherbicide by Phoma sp. in a stirred-tank bioreactor. 3 Biotech, v.6, n.2, p. 230-239, 2016. http://dx.doi.org/10.1007/s13205-016-0557-9
CAMEOTRA, S. S. Preservation of microorganisms as deposits for patent application. Biochemical and Biophysical Research Communications, v.353, n.4, p. 849-850, 2007. http://dx.doi.org/10.1016/j.bbrc.2006.12.069
CIMMINO, A.; ANDOLFI, A.; ZONNO, M.; AVOLIO, F.; SANTINI, A.; TUZI, A.; BERESTETSKYI, A.; VURRO M.; EVIDENTE, A. Chenopodolans A–C: phytotoxic furopyrans produced by Phoma Chenopodiicola, a fungal pathogen of Chenopodium Album. Phytochemistry, v.96, n.1, p. 208-213, 2013. http://dx.doi.org/10.1016/j.phytochem.2013.10.007
COSTA, E. C.; TEIXEIRA, F. F. S.; DANTAS, T. V. M.; MELO, V. S. P.; ARAÚJO, S. A. C.; ROLIM, B. N. Princípios da estocagem e preservação de amostras microbiológicas. Ciência Animal, v.19, n.2, p. 111-122, 2009.
DHINGRA, O. D.; SINCLAIR, J. B. Long-term storage of plant pathogens. In: DHINGRA, O. D.; SINCLAIR, J. B. Basic plant pathology methods. 2. ed. Boca Raton: CRC Press, 1995. p. 61-81. https://doi.org/10.1201/9781315138138-3
EVIDENTE, M.; CIMMINO, A.; ZONNO, M. C.; MAIS, M.; BERESTETSKI, A.; SANTORO, E.; SUPERCHI, S.; VURRO, M.; EVIDENTE, A. Phytotoxins produced by Phoma Chenopodiicola, a fungal pathogen of Chenopodium Album. Phytochemistry, v.117, n.1, p. 482-488, 2015. https://doi.org/10.1016/j.phytochem.2015.07.008
FERREIRA, D. F. Sisvar: a guide for its bootstrap procedures in multiple comparisons. Ciência & Agrotecnologia, v.38, n.2, p. 109-112, 2014. http://dx.doi.org/10.1590/S1413-70542014000200001
FINATTI, D.; APARECIDO, C. C. Caracterização fisiológica e comparação de diferentes métodos na preservação, em laboratório, de isolados do gênero Verticillium. Arquivos do Instituto Biológico, v.76, n.4, p. 715-720, 2009.
FONG, Y. K.; ANUAR, S.; LIM, H. P.; THAM, F. Y.; SANDERSON, F. R. A modified filter paper technique for long-term preservation of some fungal cultures. Mycologist, v.4, n.3, p. 127-130, 2000. https://doi.org/10.1016/S0269915X(00)80090-7
GIRÃO, M. D. PRADO, M. R.; BRILHANTE, R. S. N.; CORDEIRO R. A.; MONTEIRO A. J.; SIDRIM, J. J. C.; ROCHA, M. F. G. Viabilidade de cepas de Malassezia Pachydermatis mantidas em diferentes métodos de conservação. Revista da Sociedade Brasileira de Medicina Tropical, v.37, n.3, p. 229-233, 2004. http://dx.doi.org/10.1590/S0037-86822004000300007
GREEN, L. H. Practical handbook of microbiology. 2. ed. London: CRC, 2008.
GUIMARÃES, L. C. Métodos de preservação de fungos potencialmente toxicogênicos. 2011. 55 f. Dissertação (Mestrado em Ciência dos Alimentos) - Universidade Federal de Lavras, Minas Gerais, 2011.
HINZ, H. L.; SCHWARZLÄNDER, M.; GASSMANN, A.; BOURCHIER, R. S. Successes we may not have had: a retrospective analysis of selected weed biological control agents in the United States. Invasive Plant Science and Management, v.7, n.4, p. 565-579, 2014. https://doi.org/10.1614/IPSM-D-13-00095.1
HUSSAIN, H.; KOCK, I.; AL-HARRASI, A.; AL-RAWAHI, A.; ABBAS, G.; GREEN, I. R.; SHAH, A.; BADSHAH, A.; SALEEM, M.; DRAEGER, S.; SCHULZ, B.; KROHN, K. Antimicrobial chemical constituents from endophytic fungus Phoma sp. Asian Pacific Journal of Tropical Medicine, v.7, n.9, p. 699-702, 2014. https://doi.org/10.1016/S1995-7645(14)60119-X
KIM, E. L.; LI, J. L.; DANG, H. T.; HONG, J.; LEE, C. O.; KIM, D. K.; YOON, W. D.; KIM, E.; LIU, Y.; JUNG, J. H. Cytotoxic cytochalasins from the endozoic fungus Phoma sp. of the giant jellyfish Nemopilema Nomurai. Bioorganic & Medicinal Chemistry Letters, v.22, n.9, p. 5752-5752, 2012. http://dx.doi.org/10.1016/j.bmcl.2012.03.058
KLAIC, R.; SALLET, D.; FOLETTO, E. L.; JACQUES, R. J. S.; GUEDES, J. V. C.; KUHN, R. C.; MAZUTTI, M. A. Optimization of solid-state fermentation for bioherbicide production by Phoma sp. Brazilian Journal of Chemical Engineering, v.34, n.02, p. 377-384, 2017. http://dx.doi.org/10.1590/0104-6632.20170342s20150613
KOYAMA, N.; NAGAHIRO, T.; YAMAGUCHI, Y.; OHSHIRO, T.; MASUMA, R.; TOMODA, H.; OMURA, S. Spylidone, a novel inhibitor of lipid droplet accumulation in mouse macrophages produced by Phoma sp. FKI-1840. Journal of Antibiotics, v.58, n.5, p. 338-345, 2005. http://dx.doi.org/10.1038/ja.2005.42
MAIA, F. G. M.; ARMESTO, C.; ZANCAN, W. L. A.; MAIA, J. B.; ABREU, M. S. Efeito da temperatura no crescimento micelial, produção e germinação de conídios de Colletotrichum spp. isolados de mangueira com sintomas de antracnose. Bioscience Journal, v. 27, n. 2, p. 205-210, 2011.
NAKASONE, K. K.; PETERSON, A. W.; JONG, S. Preservation and distribution of fungal cultures. In: MUELLER, G. M.; BILLS, G. F.; FOSTER, M. S. Biodiversity of fungi, inventory and monitoring methods. San Diego: Elsevier Academic Press, 2004. p. 37-47. https://doi.org/10.1016/B978-012509551-8/50006-4
PASSADOR, M. M.; PIRES, G. C. C.; FINATTI, D.; APARECIDO, C. C.; FIGUEIREDO, M. B. Manutenção da viabilidade e patogenicidade de culturas mantidas na micoteca “Mário Barreto Figueiredo”. Biológico, v.72, n.1, p. 51-55, 2010.
PIRES, G. C. C.; APARECIDO, C. C.; FINATTI, D. Preservação em laboratório de fungos filamentosos por longos períodos de tempo. Biológico, v.47, n.1, p. 9-16, 2012.
QIANGQIANG, Z.; JIAJUN, W; LI, L. Storage of fungi using sterile distilled water orlyophilization: comparison after 12 years. Mycoses, v.41, n.5-6, p. 255-257, 1998. http://dx.doi.org/10.1111/j.1439-0507.1998.tb00334.x
RAI, M. DESHMUKH, P.; GADE, A.; INGLE, A.; KÖVICS, G. J.; IRINYI, L. Phoma Saccardo: distribution, secondary metabolite production and biotechnological applications. Critical Reviews in Microbiology, v.35, n.3, p. 182-196, 2009. http://dx.doi.org/10.1080/10408410902975992
SANTIAGO, C.; CHRIS FITCHETT, C.; MUNRO, M. H. G.; JALIL, J.; SANTHANAM, J. Cytotoxic and antifungal activities of 5-Hydroxyramulosin, a compound produced by an endophytic fungus isolated from Cinnamomum Mollisimum. Evidence-Based Complementary and Alternative Medicine, v.12, n.10, p. 1-6, 2012. http://dx.doi.org/10.1155/2012/689310
SOLA, M. C.; OLIVEIRA, A. P.; FEISTEL, J. C.; REZENDE, C. S. M. Manutenção de microrganismos: conservação e viabilidade. Enciclopédia Biosfera, v.8, n.14, p. 1398-1418, 2012.
TERAMOTO, A.; MARTINS, M. C.; CUNHA, M. G. Avaliação de métodos para preservação de isolados de Corynespora Cassiicola (Berk. & M.A. Curtis) C.T. Wei. Pesquisa Agropecuária Tropical, v.41, n.2, p. 296-298, 2011. https://doi.org/10.5216/pat.v41i2.12571
TODERO, I.; CONFORTIN, T. C.; LUFT, L.; BRUN, T.; UGALDE, G. A.; ALMEITA, T. C.; ARNEMANN, J. A.; ZABOT, G. L.; MAZUTTI, M. A. Formulation of a bioherbicide with metabolites from Phoma sp. Scientia Horticulturae, v.241, n.1, p. 285-292, 2018a. https://doi.org/10.1016/j.scienta.2018.07.009
TODERO, I.; CONFORTIN, T. C.; SOARES, J. F.; BRUN, T.; LUFT, L.; RABUSKE, J. E.; KUHN, R. C.; TRES, M. V.; ZABOT, G. L.; MAZUTTI, M. A. Concentration of metabolites from Phoma sp. using microfiltration membrane for increasing bioherbicidal activity. Environmental Technology, v.22, n.1, p. 1-9. 2018b. https://doi.org/10.1080/09593330.2018.1441330
VILLAVERDE, J. J.; MORÁN, B. S.; SANDÍN-ESPAÑA, P.; ALONSO-PRADOS J. L.; LÓPEZ-GOTI, C. Biopesticides in the framework of the european pesticide regulation (EC) No. 1107/2009. Pest Management Science, v.70, n.1, p. 2-5, 2014. https://doi.org/10.1002/ps.3663
WIJERATNE, E. M. K.; HE, H.; FRANZBLAU, S. G.; HOFFMAN, A. M.; GUNATILAKA, A. A. L. Phomapyrrolidones A-C, Antitubercular Alkaloids from the Endophytic Fungus Phoma sp. NRRL 46751. Journal of Natural Products, v.76, n.10, p. 1860-1865, 2013. http://dx.doi.org/10.1021/np400391p
YANO, T.; AOYAGI, A.; KOZUMA, S.; KAWAMURA, Y.; TANAKA, I.; SUZUKI, Y.; TAKAMATSU, Y.; TAKATSU, T.; INUKAI, M. Pleofungins, novel inositol phosphorylceramide synthase inhibitors from Phoma sp. SANK 13899. Journal of Antibiotics, v.60, n.2, p. 136-142, 2007. http://dx.doi.org/10.1038/ja.2007.13
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