CONTROLE BIOLÓGICO DE Meloidogyne incognita POR ISOLADOS DE ACTINOMICETOS
Keywords:
Controle biológico, Gossypium hirsutum, nematoide das galhasAbstract
The Cerrado biome has great biodiversity and also includes part of the agricultural production of the country being used for the cultivation of large crops. The nematode of the Meloidogyne incognita galls can cause significant losses in the cotton crop, as well as the fungus Sclerotinia sclerotiorum that causes damage to several crops of economic importance. Among the microorganisms with potential for phytopathogen control, actinomycetes are known for the ability to produce compounds with antimicrobial action. The objective of this work was to isolate and evaluate the antagonistic potential of actinomycetes of native soils of the Cerrado Baiano area against M. incognita and S. sclerotiorum. It was obtained 18 isolates of actinomycetes and among the tested isolates, AC. O, presented the highest percentage of immobility with 99.16% and AC. R, showed the highest percentage of mortality with 72.16% of the J2 of M. incognita. Effective control of M. incognita in cotton was not observed in vivo. However, with the rotation system with soybean, the AC isolate. G, showed a significant reduction in the number of galls and eggs in the cotton root system. With respect to the antagonism of actinobacteria against S. sclerotiorum, by the pairing of cultures, the isolate AC. M, inhibited 81.99% of the mycelial growth of this phytopathogen. The results of the present work show the potential of control of phytopathogens tested by actinomycetes, since a great part of cotton production is found in Cerrado areas and these microorganisms were isolated from this biome.
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ARAÚJO, A. E. In: Cultura do Algodão no Cerrado. Embrapa Algodão. Sistema de Produção, 2 Versão Eletrônica 2ª edição. Jun/2017. Disponível em:
BENTLEY, S. D.; CHATER, K. F.; CERDEÑO-TÁRRAGA, A. M.; CHALLIS, G. L.; THOMSON, N. R.; JAMES K. D.; HARRIS, D. E.; QUAIL, M. A.; KIESER, H.; HARPER, D.; BATEMAN, A.; BROWN, S.; CHANDRA, G.; CHEN, C. W.; COLLINS, M.; CRONIN, A.; FRASER, A.; GOBLE, A.; HIDALGO, J.; HORNSBY, T.; HOWARTH, S.; HUANG, C. H.; KIESER, T.; LARKE, L.; MURPHY, L.; OLIVER, K.; O'NEIL, S.; RABBINOWITSCH, E.; RAJANDREAM, M. A.; RUTHERFORD, K.; RUTTER, S.; SEEGER, K.; SAUNDERS, D.; SHARP, S.; SQUARES, R.; SQUARES, S.; TAYLOR, K.; WARREN, T.; WIETZORREK, A.; WOODWARD, J.; BARRELL, B. G.; PARKHILL, J.; HOPWOOD, D. A. Complete genome sequence of the model actinomycete Streptomyces coelicolor A3 (2). Nature, v.417, p.141-147, 2002. http://dx.doi.org/10.1038/417141a
BONETI, J. I.; FERRAZ, S. Modificações do método de Hussey e Barker para extração de ovos de Meloidogyne exigua em raízes de cafeeiro. Fitopatologia Brasileira, v.6, p.553, 1981.
CHARTER, K. F., HOPWOOD, D. A. Streptomyces genetics. In: GOODFELLOW, M., MORDARSKI, M., WILLIAMS, S. T. The Biology of the Actinomycetes. Academic Press, London, 1984. p.229-286.
COIMBRA, J. L.; CAMPOS, V. P. Efeito de exsudatos de colônias e de filtrados de culturas de actinomicetos na eclosão, motilidade e mortalidade de juvenis do segundo estádio de Meloidogyne javanica. Fitopatologia Brasileira, v.30, p.232-238, 2005. http://dx.doi.org/10.1590/S0100-41582005000300003
COIMBRA, J. L.; CAMPOS, V. P. Efeito antagônico de actinomicetos isolados de ervas daninhas e gramíneas na formação de galhas e na reprodução de Meloidogyne javanica em tomateiro. Revista de Biologia e Ciências da Terra, v.10, n.2, 2010.
CRAWFORD, D. L. Biodegradation of agricultural and urban wastes. In: GOODFELLOW, M.; WILLIANS, S. T.; MORDARSKY, M. Actinomycetes in Biotechnology. San Diego, Wiley Interscience, 1988. p.433-459.
CRAWFORD, D. L.; LYNCH, J. M.; WHIPPS, J. M.; OUSLEY, M. A. Isolation and characterization of actinomycete antagonist of a fungal root pathogen. Applied and environmental microbiology, v.59, n.11, p.3899-3905, 1993. https://doi.org/10.1016/B978-0-12-289673-6.50015-4
DAMASCENO, J. C. A.; SOARES, A. C. F. Controle por bactérias de nematoides que atacam a cultura do tomate. Cultivar HF, v.11, p.16-18, 2013.
FERREIRA, D. F. Sisvar: a computer statistical analysis system. Ciência e Agrotecnologia, v.35, n.6, p.1039-1042, 2011. http://dx.doi.org/10.1590/S1413-70542011000600001
HUSSEY, R. S.; BARKER, K. R. A comparison of methods for colecting inocula of Meloidogyne spp. including a new technique. Plant Disease Reporter, v.57, p.1025-1028, 1973.
INOMOTO, M. M.; SIQUEIRA, K. M. S.; MACHADO, A. C. Z. Sucessão de cultura sob pivô central para controle de fitonematoides: variação populacional, patogenicidade e estimativa de perdas. Tropical Plant Pathology, v.36, n.3, p.178-185, 2011. http://dx.doi.org/10.1590/S1982-56762011000300006
JONATHAN, E.; BARKER, K. R.; ABDEL-ALIM, F. F.; VRAIN, T. C.; DICKSON, D. W. Biological control of Meloidogyne incognita on tomato and banana with rhizobacteria, actinomycetes, and Pasteuria penetrans. Nematropica, v.30, n.2, p.231-240, 2000.
KORN-WENDISCH, F.; KÜTZNER, H. J. The family Streptomycetaceae. In: BALOWS, A.; TRÜPER, H. G.; DWORKIN, M.; SCHULEIFER, K. H. (Eds.). The prokaryotes. 2.ed. New York: Springer-Verlag. 1992. p.921-995.
LIU, X.; BOLLA, K.; ASHFORTH, E. J.; ZHUO, Y.; GAO, H.; HUANG, P.; STANLEY, S. A.; HUNG, D. T.; ZHANG, L. Systematics-guided bioprospecting for bioactive microbial natural products. Antonie Van Leeuwenhoek, v.101, p.55-66, 2012. https://doi.org/10.1007/s10482-011-9671-1
MEDEIROS, J. E.; MARIANO, R. L. R.; PEDROSA, E. M. R.; SILVEIRA, E. B. Inconsistency of the biological control of Meloidogyne incognita race 2 in melon by endophytic and rhizosphere bacteria. Horticultura Brasileira, v.27, n.3, p.319-324, 2009. http://dx.doi.org/10.1590/S0102-05362009000300010
MELO, I. S. Agentes microbianos de controle de fungos fitopatogênicos. In: MELO, I. S.; AZEVEDO, J. L. Controle biológico. Jaguariúna: Embrapa-CNPMA, 1998. p. 17-67.
SANGLIER, J. J.; WELLINGTON, E. M. H.; BEHAL, V.; FIEDLER, H. P.; GHORBEL, R. E.; FINANCE, C.; HACENE, M.; KAMOUN, A.; KELLY, C.; MERCER, D. K.; PRINZIS, S.; TRIGO, C. Novel bioactive compounds from actinomycetes. Research in Microbiology, v.144. p.661-663. 1993. https://doi.org/10.1016/0923-2508(93)90071-9
SOLTANZADEH, M.; NEJAD, M. S.; BONJAR, G. H. S. Application of soil-borne actinomycetes for biological control against Fusarium wilt of chickpea (Cicer arietinum) caused by Fusarium solani f. sp. pisi. Journal of Phytopathology, v.164, p.967-978, 2016. https://doi.org/10.1111/jph.12517
SOUSA, C. S.; SOARES, A. C. F.; GARRIDO, M. S.; ALMEIDA, G. M. C. O. Estreptomicetos no controle da meloidoginose em mudas de tomateiro. Pesquisa Agropecuária Brasileira, v.41, n.12, p.1759-1766, 2006. http://dx.doi.org/10.1590/S0100-204X2006001200010
TSAO, P. H.; LEBEN, C.; KEITT, G. W. An enrichment method for isolating actinomycetes that produce diffusible antifungal antibiotics. Phytopathology, v.50, p.88-89, 1960.
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