Caracterização genômica dos genes galactinol sintase (GolS) em Ipomea trifida: uma abordagem usando bioinformática
Keywords:
Bioinformática, melhoramento genético, batata-doce, família da rafinose (RFO)Abstract
A galactinol sintase (GolS - EC 2.4.1.123) é classificada como uma enzima chave que catalisa a primeira etapa na via de síntese da família da rafinose (RFOs). Embora os genes GolS tenham sido caracterizados em diversas espécies importantes, a sua caracterização na batata-doce ainda não foi explorada. Ipomoea trifida (Kunth) G. Don (2n = 2x = 30) é atualmente descrita como um dos ancestrais mais próximos da batata-doce e considerada uma excelente espécie de cruzamento, permitindo a introgressão de genes importantes como o GolS. Este estudo teve como objetivo identificar e caracterizar in silico os genes GolS em I. trifida e compará-los com I. triloba. Identificamos nove genes GolS, cinco em I. triloba e quatro em I. trifida. Nosso estudo abrangeu vários aspectos, incluindo análise da estrutura genética, identificação de motivos, distribuição cromossômica, análise de sintenia e expressão genica. A presença de duplicações gênicas e seleção purificadora foram destacadas, sugerindo o significado evolutivo dos genes GolS nessas espécies. A análise filogenética categorizou as proteínas GolS em três grupos, refletindo potencialmente papéis funcionais distintos. Além disso, a análise de sintenia revelou relações ortólogas entre os genes GolS nas espécies estudadas e plantas relacionadas, contribuindo para a nossa compreensão da sua história evolutiva. A análise de expressão in silico revelou padrões de expressão específicos em diferentes tecidos, sugerindo papéis especializados para genes GolS em diferentes órgãos vegetais. Estas descobertas contribuem para o campo mais amplo da genética das plantas, do metabolismo de carboidrato e da agricultura, oferecendo oportunidades para o melhoramento das culturas e a produção sustentável de alimentos.
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