Determination of the viability of Azospirillum syrups, multiplied in different culture media
DOI:
https://doi.org/10.5747/ca.2023.v19.h524Keywords:
bioinputs; Contamination; biological control;Abstract
The use of products of microbial origin, bacteria, for agriculture has grown exponentially in the world, and in Brazil this growth is significant. In recent years, several companies have settled in the country and started to develop different products in this segment. The producer has the possibility of multiplying bacteria on the farms themselves in a process called “on Farm”, a homemade way of multiplying bacteria. The objective of this work was to evaluate the efficiency of the mixtures produced through the “on Farm” method of multiplication of microorganisms from inoculants based on Azospirillum with different culture media. For this, three culture media were used: commercial product Multibacter®, yeast syrup plus sugar and yeast syrup, which were subsequently quantified. The syrups were produced in the on Farm system, the sterilization of the place of packaging and allocation of the bioreactors was carried out, the samples of the syrups were diluted for incubation and the preparation of the TSA also took place. It was possible to conclude that the non-sterile culture media do not present satisfactory results for the multiplication of on Farm microorganisms with Azospirillum syrups. Colony forming units are not parameters to indicate viability for non-sterile culture media.
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ALMEIDA, L. S.; PAIVA SOBRINHO, S.; LUZ, P. B.; CALDEIRA, D. S. A.; OLIVEIRA, A. J.; OLIVEIRA, T. C.; SILVA, G. V. B. Use of Azospirillum brasilense inoculant in the production of Tento Carolina seedlings. Research, Society and Development, v.10, n.1, 2021. https://doi.org/10.33448/rsd-v10i1.11469
ANDRADE, A.C., FONSECA, D.M., QUEIROZ, D.S., SALGADO, L.T. AND CECON, P.R. Nitrogen and potassium fertilization in elephant grass (Pennisetum purpureum Schum.cv. Napier). Science and Agrotechnology, (spec. ed.), 1643-51, 2020.
BALOTA, E. L.; HUNGARY, M.; DÖBEREINER, J. Occurrence of diazotrophic bacteria and vesicular-arbuscular mycorrhizal fungi associated with cassava (Manihot esculenta Crantz). In: INTERNATIONAL SYMPOSIUM ON NITROGEN FIXATION WITH NON-LEGUMES, 6., 1994, Ismailia. Anais [...]. Ismailia, Egypt: Cairo Univ., 1994. p.53-58. https://doi.org/10.1590/S0100-204X1999000700020
BALOTA, E. L.; LOPES, E. S.; HUNGARY, M.; DÖBEREINER, J. Occurrence of diazotrophic bacteria and arbuscular mycorrhizal fungi in cassava crops. Brazilian Agricultural Research, v.34, p.1265-1276, 1999.
BARTCHECHEN, A. et al. Effect of Azospirillum brasilense inoculation on maize (Zea mays L.) yield. Campo Digital, v. 5, n. 1, p.56-59, 2021.
BORSARI, A. P.; CLAUDINO, M. Biodefensives: market and perception of Brazilian producers. Agroanalysis. ABCBio. 2018. Disponível em: https://www.abcbio.org.br/informativos/publicacoes/
BRAZIL. Decree no. 4074, of January 4, 2002. Disponível em: http://www.planalto.gov.br/ccivil_03/decreto/2002/D4074.htm#anexoii.24
BRAZIL. Ministry of Agriculture. Manual of procedures for registration of pesticides. 2012. Available at: http://www.agricultura.gov.br/assuntos/insumos-agropecuarios/insumos- agricolas/agrotoxicos/arquivos/manual-de-procedimentos-para-registro-de-agrotoxicos.pdf
CAIRES, E. F.; ZARDO FILHO, R.; BARTH, G.; JORIS, H. A. W. Optimizing nitrogen use efficiency for no-till corn production by improving root growth and capturing NO3 N2 in subsoil. Pedosphere, v. 26, p. 474–485, 2018. https://doi.org/10.1016/S1002-0160(15)60058-3
CANTARELLA, H. Nitrogen. In: NOVAIS, R. F.; ALVAREZ V., V. H.; BARROS, N. F.; FONTES, R. L. F.; CANTARUTTI, R.B.; NEVES, J. C. L. (Ed.). Soil fertility. Viçosa: Brazilian Society of Soil Science, 2017. p.375-470.
CORREA, O. S.; ROMERO, A.M.; SORIA, M.A.; ESTRADA, M. Azospirillum brasilense-plant genotype interactions modify tomato response to bacterial diseases, and root and foliar microbial communities. In: CASSÁN, F. D.; GARCÍA DE SALAMONE, I. (ed.) Azospirillum sp.: cell physiology, plant interactions and agronomic research in Argentina. Argentina: Asociación Argentina de Microbiologia, 2018. p.87-95.
DARTORA, J.; GUIMARÃES, V. F. Nitrogen fertilization associated with inoculation with Azospirillum brasilense and Herbaspirillum seropedicae in corn. Brazilian Journal of Agricultural and Environmental Engineering, v.17, n.10, p.1023–1029, 2013.
DIAS, V. C. et al. Effects of seed inoculation with Azospirillum brasilense and nitrogen dose on oil content of corn grains. Magazine of Agriculture, v.93, n.3, 2018. https://doi.org/10.37856/bja.v93i3.3264
FERNANDES, F. C. S.; LIBARDI, P. L. Percentage of nitrogen recovery by corn, for different rates and installments of nitrogen fertilizer. Brazilian Magazine of Corn and Sorghum, v.6, n.3, p.285-296, 2017.
FRANCHE C.; LINDSTROM K.; ELMERICH C. Nitrogen-fixing bacteria associated with leguminous and non-leguminous plants. Plant and Soil, p.35–59, 2009. https://doi.org/10.1007/s11104-008-9833-8
GLICK, B. R.; TODOROVIC, B.; CZARNY, J.; CHENG, Z.; DUAN, J.; MCCONKEY, B. Promotion of plant growth by bacterial ACC deaminase. Critical Reviews in Plant Sciences, p. 227-242, 2007. https://doi.org/10.1080/07352680701572966
HUERGO, L.F. et al. Regulation of nitrogen fixation in Azospirillum brasilense. In: CASSÁN, F.D.; GARCIA DE SALAMONE, I. Azospirillum sp.: cell physiology, plant interactions and agronomic research in Argentina. Argentina: Asociación Argentina de Microbiologia, p.17-35, 2018.
HUNGARY, M.; CAMPO, R.J.; MENDES, I.C. The importance of the biological nitrogen fixation process for the soybean crop: an essential component for the competitiveness of the Brazilian product. Londrina: EMBRAPA, 2020. 80p. (Documents, 283). https://doi.org/10.1007/s11104-009-0262-0
HUNGARY, M. et al. Inoculation with selected strains of Azospirillum brasilense and A. lipoferum improves yields of maize and wheat in Brazil. Plant and Soil, v.331, n.1/2, p. 413-425, 2020.
KLOEPPER, J.W.; LIFSHITZ, R.; ZABLOTWICZ, R.M. Free-living bacterial inoculate for enhancing crop productivity. Trends in Biotechnology, v.7, p.39-43, 1989. https://doi.org/10.1016/0167-7799(89)90057-7
MILLÉO, M. V. R.; CRISTÓFOLI, I. Evaluation of the agronomic efficiency of Azospirillum sp. in the corn crop. Revista Scientia Agraria, v. 17, p. 14-23, 2019.
NEWTON, W. E. Physiology, biochemistry, and molecular biology of nitrogen fixation. In: Biology of the nitrogen cycle. Elsevier, 2007. p.109-129. https://doi.org/10.1016/B978-044452857-5.50009-6
RAMPIM, L.; GUIMARÃES, V. F.; SALLA, F. H.; COSTA, A. C. P. R.; INAGAKI, A. M.; BULEGON, L. G.; DE FRANÇA, R. Initial development of king corn seedlings inoculated with diazotrophic bacteria. Research, Society and Development, v.9, n.5, e24953109-e24953109, 2020. https://doi.org/10.33448/rsd-v9i5.3109
REINHARDT, É. L.; RAMOS, P. L.; MANFIO, G. P.; BARBOSA, H. R.; PAVAN, C.; MOREIRA-FILHO, C. A. Molecular characterization of nitrogen-fixing bacteria isolated from Brazilian agricultural plants at São Paulo State. Brazilian Journal of Microbiology, v.39, p.414-422, 2018. https://doi.org/10.1590/S1517-83822008000300002
SCHMIDELL, W.; LIMA, U. A.; AQUARONE, E.; BORZANI, W. Industrial biotechnology. Biochemical Engineering. Sao Paulo: Blucher. 2021. v.2.
SOUZA SALES, L. Z.; GARCIA, N. F. S.; MARTINS, J. T.; DE SOUZA BUZO, F.; GARÉ, L. M.; RODRIGUES, R. A. F.; ARF, O. Inoculation with Azospirillum brasilense and reduction of nitrogen fertilization in upland rice. Research, Society and Development, v.10, n.7, e9110716345-e9110716345, 2021. https://doi.org/10.33448/rsd-v10i7.16345
TEIXEIRA FILHO, M. C. M.; BUZETTI, S.; ANDREOTTI, M.; ARF, O.; BENETT, C. G. S. Rates, sources and timing of nitrogen application on irrigated wheat under no-tillage. Brazilian Agricultural Research, v.45, p.797-804, 2020. https://doi.org/10.1590/S0100-204X2010000800004
TURAN, M.; GULLUCE, M.; VON WIREN, N.; SAHIN, F. Yield promotion and phosphorus solubilization by plant growth-promoting rhizobacteria in extensive wheat production in Turkey. Journal Plant Nutrition and Soil Science, v.175, p.818–826, 2012. https://doi.org/10.1002/jpln.201200054
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