Cost reduction on micropropagation of Solanum lycopersicum L. var. cerasiform
Keywords:
alternative medium, carbon source, chemical sterilization, cherry tomato, gelling agentAbstract
The aim of this study was to establish a low cost alternative protocol for the micropropagation of Solanum lycopersicum L. var. cerasiforme. In the in vitro establishment, culture media containing P.A. and commercial sucrose and varying concentrations of corn starch and agar were tested. The replacement of physical sterilization for chemical sterilization was also evaluated. In the multiplication step, the medium was supplemented with agar and / or corn starch and commercial sucrose. For rooting, medium with commercial sucrose supplemented with agar and / or starch was used. Subsequently, the microplants were acclimatized in a greenhouse. The results allow us to conclude that the cost reduction in the micropropagation of cherry tomato is obtained by the substitution of P.A. sucrose by commercial sucrose, partial (establishment and multiplication) and total (rooting) substitution of agar by corn starch and also by the use of chemical sterilization of the medium. The microplants obtained from this protocol were successful in acclimatization step.
Downloads
References
AGRAWAL, A.; SANAYAIMA, R.; TANDON, R.; TYAGI, R.K. Cost-effective in vitro conservation of banana using alternatives of gelling agent (isabgol) and carbon source (market sugar). Acta Physiologiae Plantarum, v. 32, n. 4, p. 703–711, 2010. http://dx.doi.org/10.1007/s11738-009-0450-9
BERNARDI, W.F.; RODRIGUES, B.I.; NETO, P.C.; ANDO, A.; NETO, A.T.; CERAVOLO, L.C.; MONTES, S.N.M.N. Micropropagação de baixo custo em Bananeira cv. Maçã em meios com diferentes fontes de carbono e avaliação da performance em campo das mudas produzidas. Revista Brasileira de Fruticultura, v. 26, n. 3, p. 503–506, 2004. http://dx.doi.org/10.1590/S0100-29452004000300031
COSTA, F.H.S.; PEREIRA, M.A.A.; OLIVEIRA, J.P.; PEREIRA, J.E.S. Efeito de agentes geleificantes alternativos no meio de cultura in vitro de abacaxizeiro e bananeira. Ciência e Agrotecnologia, v. 31, n. 1, p.41–46, 2007.
ERIG, A.C., SCHUCH, M.W.; SILVA, L.D. Multiplicação in vitro de macieira (Malus domestica Borkh.) cv. Galaxy: meio de cultura e agentes solidificantes alternativos. Revista Brasileira de Agrociência, v. 10, n. 3, p. 297-302, 2004.
Ferreira, D.F. Sisvar: a computer statistical analysis system. Ciência e Agrotecnologia, v. 35, n. 6, p. 1039–1042, 2011.
GOLLE, D.P.; REINIGER, L.R.S.; CURTI, A.R.; HANAUER, J.G.; WALDOW, D.A.G. Substratos alternativos e tratamentos pré-germinativos na germinação in vitro de sementes de Pinus taeda L. Revista Árvore, v. 34, n. 1, p. 39-48, 2010. Golle, D. P., Reiniger, L. R. S., Curti, A. R., Hanauer, J. G., & Waldow, D. A. G. (2010). Substratos alternativos e tratamentos pré-germinativos na germinação in vitro de sementes de Pinus taeda L. Revista Árvore, 34(1), 39–48. http://dx.doi.org/10.1590/s0100-67622010000100005
HAMMER, O.; HARPER, D.A.T.; RYAN, P.D. PAST – Palaeontological statistics, ver. 1.89. Palaentologia Electronica, v. 4, n.1, p. 1-92, 2009.
KODYM, A.; ZAPATA-ARIAS, F.J. Low-cost alternatives for the micropropagation of banana. Plant Cell, Tissue and Organ Culture, v. 66, n. 1, p. 67–71, 2001. https://doi.org/10.1023/A:1010661521438
Labouriau, L.G. A germinação das sementes. Caracas, Venezuela: Secretaria Geral da Organização dos Estados Americanos, 1983.
LAMEIRA, O.A.; LEMOS, O.F.; MENEZES, I.C.; PINTO, J.E.B.P. Cultura de tecidos (Manual). Embrapa Amazônia Oriental, 2000.
Maguire, J.D. Speed of germination-aid in selection and evaluation for seedling emergence and vigor. Crop science, v. 2, n. 2, p. 176-177, 1962.
Murashige, T.; Skoog, F. A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiologia Plantarum, v. 15, n. 3, p. 473–497, 1962.
OLIVEIRA, R.S.; PEREIRA, M.R.; CARVALHO, V.S.; SILVA, J.R.; CAMPOSTRINI, E. Esterilização química e agentes geleificantes alternativos na propagação vegetativa in vitro do abacaxizeiro ‘Vitória’. Revista Brasileira de Fruticultura, v. 37, n. 4, p. 819–826, 2015. https://doi.org/10.1590/0100-2945-121/14
OTROSHY, M.; KHALILI, Z.; EBRAHIMI, M.A.; NEKOUI, M.K.; MORADI, K. Effect of growth regulators and explant on plant regeneration of Solanum lycopersicum L. var. cerasiforme. Russian Agricultural Sciences, v. 39, n. 3, p. 226–235, 2013. https://doi.org/10.3103/S1068367413030178
SILVA, A.C.; COSTA, C.A.; SAMPAIO, R.A.; MARTINS, E.R. Avaliação de linhagens de tomate cereja tolerantes ao calor sob sistema orgânico de produção. Revista Caatinga, v. 24, n. 3, p. 33–40, 2011.
SOLDATELI, F. J.; BATISTA, C. B.; GODOY, F.; MELLO, A. C.; DOS SANTOS SOARES, F.; BERGMANN, M. D.; ETHUR, L. Z. Crescimento e produtividade de cultivares de tomate cereja cultivadas em substratos orgânicos. Colloquium Agrariae, v. 16, n. 1, p. 1-10, 2020. https://doi.org/10.5747/ca.2020.v16.n1.a34
TEIXEIRA, S.L.; RIBEIRO, J.M.; TEIXEIRA, M.T. Influence of NaClO on nutrient medium sterilization and on pineapple (Ananas comosus cv Smooth cayenne) behavior. Plant Cell Tissue and Organ Culture, v. 86, n. 3, p. 375–378, 2006. https://doi.org/10.1007/s11240-006-9121-3
TIWARI, A.K.; TRIPATHI, S.; LAL, M.; MISHRA, S. Screening of some chemical disinfectants for media sterilization during in vitro micropropagation of sugarcane. Sugar Tech, v. 14, n. 4, p.364-369, 2012. https://doi.org/10.1007/s12355-012-0178-5
TYAGI, R.K.; AGRAWAL, A.; MAHALAKSHMI, C.; HUSSAIN, Z.; TYAGI, H. Low-cost media for in vitro conservation of turmeric (Curcuma longa L.) and genetic stability assessment using RAPD markers. In Vitro Cellular and Developmental Biology – Plant, v. 43, n. 1, p. 51–58, 2007. https://doi.org/10.2307/20461644
Downloads
Published
Issue
Section
License
Copyright (c) 2021 Colloquium Agrariae. ISSN: 1809-8215
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.