CHARACTERIZATION OF LIMA BEAN NODULATING RHIZOBIA IN SOIL FROM THREE STATES OF BRAZILIAN NORTHEASTERN
Keywords:
Phaseolus lunatus, leguminous, tropical soilsAbstract
The aim of this study was to characterize lima bean nodulating rhizobial from soils of northeastern Brazil. Soil samples were collected in regions with production of lima bean in the Ceara, Piaui and Maranhao states. The experiment was conducted in pots containing soil from each region, sowed with lima bean. Nodules were sampled for isolation and characterization at 35 days after plant emergence. A total of 75 isolates were found and submitted to morphological and physiological characterization. Morphologically, the majority of isolates exhibited circular and convex colonies with entire border and smooth surface. Physiologically, all isolated presented fast growth, and the majority of isolates presented acid reaction, moderate ability to produce mucus with gummy consistency and colonies with cream coloration. The isolates found in this study presented similar characteristics to genera Rhizobium, Sinorhizobium,Allorhizobium eMesorhizobium.
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BARAÚNA, A.C., SILVA, K., PEREIRA, G.M.D., KAMINSKI, P.E., PERIN, L., ZILLI, J.E. Diversity and nitrogen fixation efficiency of rhizobia isolated from nodules of Centrolobium paraense. Pesquisa Agropecuária Brasileira, v. 49, n. 4, p. 296-305, 2014. http://dx.doi.org/10.1590/S0100-204X2014000400008
BEZERRA, B.R.; ARAÚJO, A.S.F.; SANTOS, J.A.; CARNEIRO, R.F.V. Fertilidade do solo e adubação. In: ARAÚJO, A.S.F.; LOPES, Â.C.A.; GOMES, R.L.F. (Org.). A cultura do feijão-fava na Região Meio-Norte do Brasil. 1 ed. Teresina: EDUFPI, 2010. v.1, p.129-138.
CARDOSO, A.A., ANDRAUS, M.P., BORBA, T.C.O., MARTIN-DIDONET, C.C.G., FERREIRA, E.P.B. Characterization of rhizobia isolates obtained from nodules of wild genotypes of common bean. Brazilian Journal of Microbiology, v. 48, n. 1, p. 43-50, 2017. https://doi.org/10.1016/j.bjm.2016.09.002
CHAGAS JÚNIOR, A.F.; OLIVEIRA, L.A.; CASTRO, H.G.; CORNÉLIO, G.L.; SANTOS, G.R.; CHAGAS, L.F.B.; COSTA, J.L. Isolation and phenotypic characterization of rhizobia that nodulate cowpea in the Cerrado in Tocantins State, Brazil. Journal of Biotechnology and Biodiversity, v.4, n.3, 249-259, 2013. https://repositorio.inpa.gov.br/bitstream/123/4787/1/isolation.pdf 21 Março 2019.
CRUZ, C.D.; REGAZZI, A.J.; CARNEIRO, P.C.S. Modelos biométricos aplicados ao melhoramento genético. Viçosa: UFV. 480p. 2004. http://www.sidalc.net/cgi-bin/wxis.exe/?IsisScript=agrono.xis&method=post&formato=2&cantidad=1&expresion=mfn=005438 21 Março 2019.
CRUZ, C. D. GENES – a software package for analysis in experimental statistics and quantitative genetics. Acta Scientiarum, v.35, p. 271-276, 2013. http://dx.doi.org/10.4025/actasciagron.v35i3.21251
DABESSA, A.; ABEBE, Z.; BEKELE, S. Limitations and strategies to enhance biological nitrogen fixation in sub-humid tropics of Western Ethiopia. Journal of Agricultural Biotechnology and Sustainable Development, v. 10, n. 7, p. 122-131, 2018. http://dx.doi.org/10.5897/JABSD2018.0318
DURÁN, D., REY, L., MAYO, J., ZÚÑIGA-DÁVILA, D., IMPERIAL, J., RUIZ-ARGÜESO, T., MARTI´NEZ-ROMERO, E., ORMEÑO-ORRILLO, E. Bradyrhizobium paxllaeri sp. nov. and Bradyrhizobium icense sp. nov., nitrogen-fixing rhizobial symbionts of Lima bean (Phaseolus lunatus L.) in Peru. International journal of systematic and evolutionary microbiology, v. 64, n. 6, p. 2072-2078, 2014. http://dx.doi.org/10.1099/ijs.0.060426-0
HUNGRIA, M. Coleta de nódulos e isolamento de rizóbio. In: HUNGRIA, M.; ARAUJO, R.S. (Eds.) Manual de métodos empregados em estudo de microbiologia agrícola. Brasília: Embrapa-spi, 1994. p. 45-61.
HUNGRIA, M.; O'HARA, G.; ZILLI, J., ARAUJO, R.; DEAKER, R.; HOWIESON, J. Isolation and growth of rhizobia. In: Howieson JG, Dilworth MJ (eds) Isolation and growth of rhizobia. Australian Centre for International Agricultural Research (ACIAR), Canberra, p. 39–60. 2016. http://researchrepository.murdoch.edu.au/id/eprint/34579 21 Março 2019.
LÓPEZ-LÓPEZ, A.; NEGRETE-YANKELEVICH, S.; ROGEL, M.A.; ORMEÑO-ORRILLO, E.; MARTÍNEZ, J.; MARTÍNEZ-ROMERO, E. Native bradyrhizobia from Los Tuxtlas in Mexico are symbionts of Phaseolus lunatus (Lima bean). Systematic and applied microbiology, v. 36, n. 1, p. 33-38, 2013. https://doi.org/10.1016/j.syapm.2012.10.006
MARCHETTI, M.M.; SANTOS, J.C.P.; BARATTO, C.M. Caracterização de bactérias em nódulos de leguminosas arbóreas de fragmentos da floresta ombrófila mista. Scientia agraria, v. 18, n. 4, p. 50-62, 2017. https://dialnet.unirioja.es/servlet/articulo?codigo=6294990 21 Março 2019.
MARTINS, L.M.V.; XAVIER, G.R.; NEVES, M.C.P.; RUMJANEK, N.G. Características relativas ao crescimento em meio de cultura e a morfologia de colônias de “Rizóbio”. Embrapa Agrobiologia-Comunicado Técnico (INFOTECA-E). 14p, 1997. https://www.infoteca.cnptia.embrapa.br/bitstream/doc/623573/1/cot019.pdf 21 Março 2019.
MATSUBARA, M.; ZÚÑIGA-DÁVILA, D. Phenotypic and molecular differences among rhizobia that nodulate Phaseolus lunatus in the Supe valley in Peru. Annals of microbiology, v. 65, n. 3, p. 1803-1808, 2015. http://dx.doi.org/10.1007/s13213-015-1054-9
MELLONI, R.; MOREIRA, F.M.D.S.; NÓBREGA, R.S.M.; SIQUEIRA, J.O. Eficiência e diversidade fenotípica de bactérias diazotróficas que nodulam caupi [Vigna unguiculata(L.) Walp] e feijoeiro (Phaseolus vulgaris L.) em solos de mineração de bauxita em reabilitação. Revista Brasileira de Ciência do Solo, v.30, n.2, p.235-246, 2006. https://www.redalyc.org/html/1802/180214051005/ 21 Março 2019.
MOTTA-ALDANA, J. R., SERRANO-SERRANO, M. L., HERNÁNDEZ-TORRES, J., CASTILLO-VILLAMIZAR, G., DEBOUCK, D. G. Multiple origins of Lima bean landraces in the Americas: Evidence from chloroplast and nuclear DNA polymorphisms. Crop Science, v. 50, n. 5, p. 1773-1787, 2010. http://dx.doi.org/10.2135/cropsci2009.12.0706
ORMEÑO, E.; TORRES, R.; MAYO, J.; RIVAS, R.; PEIX, A.; VELÁZQUEZ, E.; ZÚNIGA, D. Phaseolus lunatus is nodulated by a phosphate solubilizing strain of Sinorhizobium meliloti in a Peruvian soil. In: First International Meeting on Microbial Phosphate Solubilization. Springer, Dordrecht, p. 143-147. 2007. https://link.springer.com/chapter/10.1007/978-1-4020-5765-6_21 21 Março 2019.
ORMENO-ORRILLO, E.; VINUESA, P.; ZUNIGA-DAVILA, D.; MARTÍNEZ-ROMERO, E. Molecular diversity of native bradyrhizobia isolated from Lima bean (Phaseolus lunatus L.) in Peru. Systematic and applied microbiology, v. 29, n. 3, p. 253-262, 2006. https://doi.org/10.1016/j.syapm.2005.09.002
RODRIGUES, D.R.; SILVA, A.F.; CAVALCANTI, M.I.P.; ESCOBAR, I.E.C.; FRAIZ, A.C.R.; RIBEIRO, P.R.A.; FERREIRA NETO, R.A.; FREITAS, A.D.S.; FERNANDES-JÚNIOR, P.I. Phenotypic, genetic and symbiotic characterization of Erythrina velutina rhizobia from Caatinga dry forest. Brazilian Journal of Microbiology, v. 49, n. 3, p. 503-512, 2018. http://dx.doi.org/10.1016/j.bjm.2017.09.007
RUFINI, M.; FERREIRA, P. A. A.; SOARES, B. L.; OLIVEIRA, D. P.; ANDRADE, M. J. B.; MOREIRA, F. M. S. Simbiose de bactérias fixadoras de nitrogênio com feijoeiro-comum em diferentes valores de pH. Pesquisa Agropecuária Brasileira. v. 46, n.1, p.81-88, 2011.
http://seer.sct.embrapa.br/index.php/pab/article/view/9429/6186 21 Março 2019.
SANTOS, J.O.; ANTUNES, J.E.L.; ARAÚJO, A.S.F.; LYRA, M.C.C.P.; GOMES, R.L. F.; LOPES, A.C.A.; FIGUEIREDO, M.V.B. Genetic diversity among native isolates of rhizobia from Phaseolus lunatus. Annals of Microbiology, v. 61, n. 3, p. 437-444, 2011. http://dx.doi.org/10.1007/s13213-010-0156-7
SILVA, V.N.; SILVA, L. E.D.S.F.; FIGUEIREDO, M.D.V.B.; CARVALHO, F.G.; SILVA, M.L.R.B.; SILVA, A.J.N. Caracterização e seleção de populações nativas de rizóbios de solo da região semi-árida de Pernambuco. Pesquisa Agropecuária Tropical, v.37, n.1, p.16-21, 2007. https://www.redalyc.org/html/2530/253020279004/ 21 Março 2019.
VINCENT, J.M. A manual for the pratical study of root-nodule-bacteria. Oxford, Blackwells Scientific Publications, 1970. 164p.
ANDREWS, M.; ANDREWS, M.E. Specificity in legume-rhizobia symbioses. International Journal of Molecular Sciences, v. 18, n. 4, p. 705, 2017. https://doi.org/10.3390/ijms18040705
ARAÚJO, A.S.F.; LOPES, Â.C.A.; TERAN, J.C.B.Y.; PALKOVIC, A.; GEPTS, P. Nodulation ability in different genotypes of Phaseolus lunatus by rhizobia from California agricultural soils. Symbiosis, v. 73, p. 7-14, 2017. http://dx.doi.org/10.1007/s13199-016-0465-0
BARAÚNA, A.C., SILVA, K., PEREIRA, G.M.D., KAMINSKI, P.E., PERIN, L., ZILLI, J.E. Diversity and nitrogen fixation efficiency of rhizobia isolated from nodules of Centrolobium paraense. Pesquisa Agropecuária Brasileira, v. 49, n. 4, p. 296-305, 2014. http://dx.doi.org/10.1590/S0100-204X2014000400008
BEZERRA, B.R.; ARAÚJO, A.S.F.; SANTOS, J.A.; CARNEIRO, R.F.V. Fertilidade do solo e adubação. In: ARAÚJO, A.S.F.; LOPES, Â.C.A.; GOMES, R.L.F. (Org.). A cultura do feijão-fava na Região Meio-Norte do Brasil. 1 ed. Teresina: EDUFPI, 2010. v.1, p.129-138.
CARDOSO, A.A., ANDRAUS, M.P., BORBA, T.C.O., MARTIN-DIDONET, C.C.G., FERREIRA, E.P.B. Characterization of rhizobia isolates obtained from nodules of wild genotypes of common bean. Brazilian Journal of Microbiology, v. 48, n. 1, p. 43-50, 2017. https://doi.org/10.1016/j.bjm.2016.09.002
CHAGAS JÚNIOR, A.F.; OLIVEIRA, L.A.; CASTRO, H.G.; CORNÉLIO, G.L.; SANTOS, G.R.; CHAGAS, L.F.B.; COSTA, J.L. Isolation and phenotypic characterization of rhizobia that nodulate cowpea in the Cerrado in Tocantins State, Brazil. Journal of Biotechnology and Biodiversity, v.4, n.3, 249-259, 2013. https://repositorio.inpa.gov.br/bitstream/123/4787/1/isolation.pdf 21 Março 2019.
CRUZ, C.D.; REGAZZI, A.J.; CARNEIRO, P.C.S. Modelos biométricos aplicados ao melhoramento genético. Viçosa: UFV. 480p. 2004. http://www.sidalc.net/cgi-bin/wxis.exe/?IsisScript=agrono.xis&method=post&formato=2&cantidad=1&expresion=mfn=005438 21 Março 2019.
CRUZ, C. D. GENES – a software package for analysis in experimental statistics and quantitative genetics. Acta Scientiarum, v.35, p. 271-276, 2013. http://dx.doi.org/10.4025/actasciagron.v35i3.21251
DABESSA, A.; ABEBE, Z.; BEKELE, S. Limitations and strategies to enhance biological nitrogen fixation in sub-humid tropics of Western Ethiopia. Journal of Agricultural Biotechnology and Sustainable Development, v. 10, n. 7, p. 122-131, 2018. http://dx.doi.org/10.5897/JABSD2018.0318
DURÁN, D., REY, L., MAYO, J., ZÚÑIGA-DÁVILA, D., IMPERIAL, J., RUIZ-ARGÜESO, T., MARTI´NEZ-ROMERO, E., ORMEÑO-ORRILLO, E. Bradyrhizobium paxllaeri sp. nov. and Bradyrhizobium icense sp. nov., nitrogen-fixing rhizobial symbionts of Lima bean (Phaseolus lunatus L.) in Peru. International journal of systematic and evolutionary microbiology, v. 64, n. 6, p. 2072-2078, 2014. http://dx.doi.org/10.1099/ijs.0.060426-0
HUNGRIA, M. Coleta de nódulos e isolamento de rizóbio. In: HUNGRIA, M.; ARAUJO, R.S. (Eds.) Manual de métodos empregados em estudo de microbiologia agrícola. Brasília: Embrapa-spi, 1994. p. 45-61.
HUNGRIA, M.; O'HARA, G.; ZILLI, J., ARAUJO, R.; DEAKER, R.; HOWIESON, J. Isolation and growth of rhizobia. In: Howieson JG, Dilworth MJ (eds) Isolation and growth of rhizobia. Australian Centre for International Agricultural Research (ACIAR), Canberra, p. 39–60. 2016. http://researchrepository.murdoch.edu.au/id/eprint/34579 21 Março 2019.
LÓPEZ-LÓPEZ, A.; NEGRETE-YANKELEVICH, S.; ROGEL, M.A.; ORMEÑO-ORRILLO, E.; MARTÍNEZ, J.; MARTÍNEZ-ROMERO, E. Native bradyrhizobia from Los Tuxtlas in Mexico are symbionts of Phaseolus lunatus (Lima bean). Systematic and applied microbiology, v. 36, n. 1, p. 33-38, 2013. https://doi.org/10.1016/j.syapm.2012.10.006
MARCHETTI, M.M.; SANTOS, J.C.P.; BARATTO, C.M. Caracterização de bactérias em nódulos de leguminosas arbóreas de fragmentos da floresta ombrófila mista. Scientia agraria, v. 18, n. 4, p. 50-62, 2017. https://dialnet.unirioja.es/servlet/articulo?codigo=6294990 21 Março 2019.
MARTINS, L.M.V.; XAVIER, G.R.; NEVES, M.C.P.; RUMJANEK, N.G. Características relativas ao crescimento em meio de cultura e a morfologia de colônias de “Rizóbio”. Embrapa Agrobiologia-Comunicado Técnico (INFOTECA-E). 14p, 1997. https://www.infoteca.cnptia.embrapa.br/bitstream/doc/623573/1/cot019.pdf 21 Março 2019.
MATSUBARA, M.; ZÚÑIGA-DÁVILA, D. Phenotypic and molecular differences among rhizobia that nodulate Phaseolus lunatus in the Supe valley in Peru. Annals of microbiology, v. 65, n. 3, p. 1803-1808, 2015. http://dx.doi.org/10.1007/s13213-015-1054-9
MELLONI, R.; MOREIRA, F.M.D.S.; NÓBREGA, R.S.M.; SIQUEIRA, J.O. Eficiência e diversidade fenotípica de bactérias diazotróficas que nodulam caupi [Vigna unguiculata(L.) Walp] e feijoeiro (Phaseolus vulgaris L.) em solos de mineração de bauxita em reabilitação. Revista Brasileira de Ciência do Solo, v.30, n.2, p.235-246, 2006. https://www.redalyc.org/html/1802/180214051005/ 21 Março 2019.
MOTTA-ALDANA, J. R., SERRANO-SERRANO, M. L., HERNÁNDEZ-TORRES, J., CASTILLO-VILLAMIZAR, G., DEBOUCK, D. G. Multiple origins of Lima bean landraces in the Americas: Evidence from chloroplast and nuclear DNA polymorphisms. Crop Science, v. 50, n. 5, p. 1773-1787, 2010. http://dx.doi.org/10.2135/cropsci2009.12.0706
ORMEÑO, E.; TORRES, R.; MAYO, J.; RIVAS, R.; PEIX, A.; VELÁZQUEZ, E.; ZÚNIGA, D. Phaseolus lunatus is nodulated by a phosphate solubilizing strain of Sinorhizobium meliloti in a Peruvian soil. In: First International Meeting on Microbial Phosphate Solubilization. Springer, Dordrecht, p. 143-147. 2007. https://link.springer.com/chapter/10.1007/978-1-4020-5765-6_21 21 Março 2019.
ORMENO-ORRILLO, E.; VINUESA, P.; ZUNIGA-DAVILA, D.; MARTÍNEZ-ROMERO, E. Molecular diversity of native bradyrhizobia isolated from Lima bean (Phaseolus lunatus L.) in Peru. Systematic and applied microbiology, v. 29, n. 3, p. 253-262, 2006. https://doi.org/10.1016/j.syapm.2005.09.002
RODRIGUES, D.R.; SILVA, A.F.; CAVALCANTI, M.I.P.; ESCOBAR, I.E.C.; FRAIZ, A.C.R.; RIBEIRO, P.R.A.; FERREIRA NETO, R.A.; FREITAS, A.D.S.; FERNANDES-JÚNIOR, P.I. Phenotypic, genetic and symbiotic characterization of Erythrina velutina rhizobia from Caatinga dry forest. Brazilian Journal of Microbiology, v. 49, n. 3, p. 503-512, 2018. http://dx.doi.org/10.1016/j.bjm.2017.09.007
RUFINI, M.; FERREIRA, P. A. A.; SOARES, B. L.; OLIVEIRA, D. P.; ANDRADE, M. J. B.; MOREIRA, F. M. S. Simbiose de bactérias fixadoras de nitrogênio com feijoeiro-comum em diferentes valores de pH. Pesquisa Agropecuária Brasileira. v. 46, n.1, p.81-88, 2011.
http://seer.sct.embrapa.br/index.php/pab/article/view/9429/6186 21 Março 2019.
SANTOS, J.O.; ANTUNES, J.E.L.; ARAÚJO, A.S.F.; LYRA, M.C.C.P.; GOMES, R.L. F.; LOPES, A.C.A.; FIGUEIREDO, M.V.B. Genetic diversity among native isolates of rhizobia from Phaseolus lunatus. Annals of Microbiology, v. 61, n. 3, p. 437-444, 2011. http://dx.doi.org/10.1007/s13213-010-0156-7
SILVA, V.N.; SILVA, L. E.D.S.F.; FIGUEIREDO, M.D.V.B.; CARVALHO, F.G.; SILVA, M.L.R.B.; SILVA, A.J.N. Caracterização e seleção de populações nativas de rizóbios de solo da região semi-árida de Pernambuco. Pesquisa Agropecuária Tropical, v.37, n.1, p.16-21, 2007. https://www.redalyc.org/html/2530/253020279004/ 21 Março 2019.
VINCENT, J.M. A manual for the pratical study of root-nodule-bacteria. Oxford, Blackwells Scientific Publications, 1970. 164p.
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CHARACTERIZATION OF LIMA BEAN NODULATING RHIZOBIA IN SOIL FROM THREE STATES OF BRAZILIAN NORTHEASTERN. (2019). Colloquium Agrariae. ISSN: 1809-8215, 15(6), 11-20. https://journal.unoeste.br/index.php/ca/article/view/3106