PERFIL QUÍMICO E ATIVIDADES ANTIOXIDANTE E ANTIFÚNGICA DO ÓLEO ESSENCIAL DA FLOR DE COCHLOSPERMUM REGIUM SCHRANK

Authors

  • Antonio Carlos Pereira de Menezes Filho Instituto Federal Goiano/Mestrado
  • Wendel Cruvinel de Sousa Instituto Federal de Educação, Ciência e Tecnologia Goiano
  • Marcela Christofoli Universidade Federal de Goiás, Campus Samambaia, Goiânia
  • Carlos Frederico de Souza Castro Instituto Federal de Educação, Ciência e Tecnologia Goiano

DOI:

https://doi.org/10.5747/ca.v16i4.3066

Keywords:

Cochlospermum regium; DPPH; hydrodistillation; essential oil.

Abstract

In the Cerrado domain we can find numerous plant species that can produce essential oils as second-order metabolites, such as Cochlospermum regium, popularly known as “algodãozinho-do-cerrado”. The objective of this study was to characterize the chemical composition and to evaluate the antioxidant activity of essential oil extracted from C. regium flowers. The flowers of C. regium were collected in an area of Cerrado, in Rio Verde/GO, Brazil. The species was identified by the first author and an exsicata deposited in the herbarium (IF Goiano). To extract the essential oil, 100g of flowers were used and hydrodistilled in Clevenger type apparatus. The yield of the essential oil was determined. The essential oil was analyzed by gas chromatograph associated with mass spectrometry (CG-EM) and its antioxidant potential was evaluated by the free radical sequestration technique DPPH. The average yield of essential oil was 0.099%. During GC-MS analysis, 32 compounds were identified, including caryophyllene E (9.76%), γ-muurolene (16.69%) and bicyclogermacrene (39.82%). The antioxidant activity presented concentrations between 50 and 2 mg mL-1 100% reduction of the DPPH radical, and reductions between 1 and 0.031 mg mL-1 of 85.77 and 13.18% (EC50). It is concluded that the essential oil of C. regium showed low extraction yield, however rich in hydrocarbon and oxygenated sesquiterpene compounds, and as an exceptional antioxidant and natural antifungal agent in the control of Sclerotinia sclerotiorum. Thus, the essential oil of C. regium flowers presents a strong candidate for new research that can be applied in the health, agricultural and food areas.

Downloads

Download data is not yet available.

Author Biography

  • Antonio Carlos Pereira de Menezes Filho, Instituto Federal Goiano/Mestrado
    Mestrando em Agroquímica pelo IFGoiano - Campus Rio Verde, GO, Brasil Biólogo pela Universidade de Rio Verde, Campus Rio Verde, GO, Brasil

References

ADAMS, R. P. Identification of essential oil componentes by gas chromatography/mass spectrometry. 4th, Ed. Carol Stream: Allured Publ., 2007. p. 804.
ANDRADE, M. A.; CARDOSO, M. D. A. S. G.; BATISTA, L. R.; MALLET, A. C. T.; MACHADO, S. M. F. Essential oils of Cinnamomum zeylanicum, Cymbopogon nardus and Zingiber officinale: composition, antioxidant and Antibacterial activities. Revista Ciência Agronômica, v.43, n.2, p. 399-408, 2012. http://dx.doi.org/10.1590/S1806-66902012000200025
ANDRADE, L. S.; SANTOS, D. B.; CASTRO, D. B.; GUILLO, L. A.; CHEN-CHEN, L. Absence of antimutagenicity of Cochlospermum regium (Mart. and Schr.) Pilger 1924 by micronucleus test in mice. Brazilian Journal Biology, v.68, n.1, p. 155-59, 2008. http://dx.doi.org/10.1590/S1519-69842008000100022
BARBOSA, C. S.; PEREIRA, R. F.; FORTUNA, J. L. Antifungal activity of the essential oil of Lippia alba (Mill.) N. E. Brown (Verbenaceae) against Candida albicans. Revista Biociência, v.23, n.1, p. 53-60, 2017.
BAZDAR, M.; SADEGHI, H.; HOSSEINI, S. Evaluation of oil profiles, total phenols and phenolic compounds in Prangos ferulacea leaves and flowers and their effects on antioxidant activities. Biocatalysis and Agricultural Biotechnology, v.14, p. 418-23, 2018. https://doi.org/10.1016/j.bcab.2018.04.009
BOMFIM, N. S.; NAKASSUGI, L. P.; OLIVEIRA, J. F. P.; KOHIYAMA, C. Y.; MOSSINI, S. A. G.; GRESPAN, R.; NERILO, S. B.; MALLMAN, C. A.; ABREU FILHO, B. A.; MACHINSKI JÚNIOR, M. Antifungal activity and Inhibition of fumonisin production by Rosmarinus officinalis L. essential oil in Fusarium verticillioides (Sacc.) Nirenberg. Food Chemistry, v.166, n.1, p. 330-336, 2015. https://doi.org/10.1016/j.foodchem.2014.06.019
BRUM, R. L.; HONDA, N. K.; HESS, S. C.; CRUZ, A. B.; MORETTO, E. Antibacterial activity of Cochlospermum regium essential oil. Fitoterapia, v.68, n.1, p. 79, 1997.
CAMILLO, J.; SCHERWINSKI-PEREIRA, J. E.; VIEIRA, R. F.; PEIXOTO, J. R. In vitro Conservation of Cochlospermum regium (Schrank) Pilg.- Cochlospermaceae under minimal growth storage. Revista Brasileira de Plantas Medicinais, v.11, n.2, p. 184-89, 2009.
CAMILLO, J.; MARTINS, R. C.; NETO, G. G.; GULIAS, A. P. S. M.; FILGUEIRAS, A. D.; DA COSTA, L. C.; DA SILVA, D. B. Cochlospermum regium (Algodão-do-campo). Espécies Nativas da Flora Brasileira de Valor Econômico Atual ou Potencial: Plantas para o futuro – Região Centro-Oeste. Ed., MMA, Ministério do Meio Ambiente, Brasília – DF. 2016. Cap. 5, p. 717-30.
DE CERQUEIRA, M. D.; MARQUES, E. E. J.; MARTINS, D.; ROQUE, N. F.; CRUZ, F. G.; GUEDES, M. L. D. A. S. Seasonal variation of the composition of essential oil from Myrcia salzmannii Berg. (Myrtaceae). Química Nova, v.32, n.6, p. 1544-48, 2009. http://dx.doi.org/10.1590/S0100-40422009000600035
DE MORAIS, S. M.; JÚNIOR, F. E. A. C.; DA SILVA, A. R. A.; NETO, J. S. M. Atividade antioxidante de óleos essenciais de espécies de Croton do nordeste do Brasil. Química Nova, v.29, n.5, p. 907-10, 2006.
ESMAELI, H.; KARAMI, A.; MAGGI, F. Essential oil composition, total phenolic and flavonoids contents, and antioxidant activity of Oliveria decumbens Vent. (Apiaceae) at different phenological stages. Journal of Cleaner Production, v.198, p. 91-95, 2018. https://doi.org/10.1016/j.jclepro.2018.07.029
GARCIA, R. Á.; JULIATTI, F. C.; BARBOSA, K. A. G.; CASSEMIRO, T. A. Atividade antifúngica de óleo e extratos vegetais sobre Sclerotinia sclerotiorum. Bioscience Journal, v.28, n.1, p. 48-57, 2012.
JUIZ, P. J. L.; DA SILVA, F.; CAMPOS, M. J. A.; UETANABARO, A. P. T.; ALVES, R. J. C.; LUCCHESE, A. M. Antimicrobial activity of essential oil of Ocimum americanum and Ocimum basilicum against periodontopathogens. Brazilian Journal Periodontology, v.26, n.4, p. 7-14, 2016.
JULIATTI, F. C.; CRATO, F. F.; JULIATTI, F. C.; COUTO, K. R.; JULIATTI, B. C. M. Escala diagramática para avaliação da severidade de mofo branco em soja. Bioscience Journal, v.29, n.3, p. 676-680, 2013.
KUMAR, V. MATHELA, C. S.; TEWARI, G.; SINGH, D.; TEWARI, A. K.; BISHT, K. S. Chemical composition and antifungal activity of essential oils from three Himalayan erigeron species. LWT – Food Science and Technology, v.56, n.2, p. 278-283, 2014. https://doi.org/10.1016/j.lwt.2013.12.007
MENDONÇA, R. C.; FELFILI, J, M.; WALTER, B. M. T.; SILVA JÚNIOR, M. C.; REZENDE, A. V.; FILGUEIRAS, T. S.; NOGUEIRA, P. E. 1998. Flora Vascular do Cerrado. Pp. 287-556. In: S. M. Sano & S. P. Almeida (Eds.). Cerrado: ambiente e flora. Embrapa Cerrados, Planaltina.
MEZZA, G. N.; BORGARELLO, A. V.; GROSSO, N. R.; FERNANDEZ, H.; PRAMPARO, M. C.; GAYOL, M. F. Antioxidant activity of rosemary essential oil fractions obtained by molecular distillation and their effect on oxidative stability of sunflower oil. Food Chemistry, v.242, n.1, p. 9-15, 2018. https://doi.org/10.1016/j.foodchem.2017.09.042
NASCIMENTO, M. N. G.; JUNQUEIRA, J. G. M.; TEREZAN, A. P.; SEVERINO, R. P.; SILVA, T. S.; MARTINS, C. H. G.; SEVERINO, V. G. P. Chemical composition and antimicrobial activity of essential oils from Xylopia aromatica (Annonaceae) flowers and leaves. Revista Virtual de Química, v. 10, n. 5, p. 1578-1590, 2018.
NETO, G. G.; DE MORAIS, R. G. Recursos medicinais de espécies do Cerrado de Mato Grosso: um estudo bibliográfico. Acta Botanica Brasílica, v.17, n.4, p. 561-584, 2003.
ROUQUAYROL, M. Z.; FONTELES, M. C.; ALENCAR, J. E.; ABREU-MATOS, F. J. D.; CRAVEIRO, A. A. Atividade moluscicida de óleos essenciais de plantas do nordeste brasileiro. Revista Brasileira de Pesquisa Médica Biológica, v.13, n.4-6, p. 135-43, 1980.
SILVA, E. A. J.; DA SILVA, V. P.; ALVES, C. C. F.; ALVES, J. M.; SOUCHIE, E. L.; BARBOSA, L. C. A. Chemical composition of the essential oil of Psidium guajava leaves and its toxicity against Sclerotinia sclerotiorum. Semina: Ciências Agrárias, v. 39, n. 2, p. 865-874, 2018.
SÓLON, S.; CAROLLO, C. A.; BRANDÃO, L. G.; DE MACEDO, C. D. O. S. S.; KLEIN, A.; DIAS-JÚNIOR, C. A.; DE SIQUEIRA, J. M. Phenolic derivatives and other chemical compounds from Cochlospermum regium. Química Nova, v.35, n.6, p. 1169-72, 2012. http://dx.doi.org/10.1590/S0100-40422012000600019
SÓLON, S.; BRANDÃO, L. F. G.; DE SIQUEIRA, J. M. Genus Cochlospermum Kunth with emphasis on ethnobotanic, pharmacological, toxicological, and chemical aspects of the Cochlospermum regium (Mart. et Schr.) Pilger. Revista Eletrônica de Farmácia, v.4, n.3, p. 1-22, 2009.
SOUHAIEL, N.; SIFAOUI, I.; HASSINE, D. B.; BLETON. J.; BONOSE, M.; MOUSSA, F.; PIÑERO, J. E.; LORENZO-MORALES, J.; ABDERRABBA, M. Ammoides pusilla (Apiaceae) essential oil: acitivity against Acanthamoeba castellanii Neff. Experimental Parasitology, v.183, p. 99-103, 2017. https://doi.org/10.1016/j.exppara.2017.10.011

Published

2020-07-28

How to Cite

PERFIL QUÍMICO E ATIVIDADES ANTIOXIDANTE E ANTIFÚNGICA DO ÓLEO ESSENCIAL DA FLOR DE COCHLOSPERMUM REGIUM SCHRANK. (2020). Colloquium Agrariae. ISSN: 1809-8215, 16(4), 89-101. https://doi.org/10.5747/ca.v16i4.3066