ACETOINE AND CHITINASE PRODUCTION BY NATIVE RIZOBACTERIA FROM WESTERN PARANÁ
Palavras-chave:
biocontrol; chitin degradation; PGPB.Resumo
The objective of this study was to investigate 28 strains of native rhizobacteria from the west region of Paraná, evaluating the acetoin and chitinase production capacity, because of their role in biocontrol. To quantify the acetoin production capacity, the isolates were submitted to the modified Voges-Proskauer (VP) assay in Clark & Lubs liquid medium for glucose fermentation. Metabolite production was evaluated in a spectrophotometer and it showed a wide variation in concentrations (0,476 to 1,865) for different isolates. Strains 241, 320, 326, and 273 showed higher values. According to positive results in the qualitative test of VP, 11 isolates were chosen for the tests in minimal medium containing chitin as the only source of carbon. Isolates that presented the degradation halo formation were considered as chitinolytic bacteria, evidencing chitinase activity. Among these strains, two belonged to the genus Bacillus spp. (56 and 121) and two to the genus Enterobacter spp. (292 and 151). Therefore, the present work was able to identify native strains with potential for biocontrol studies allowing to continue investigations of other metabolites produced by those bacteria in this collection regarding plant protection.
Downloads
Os dados de download ainda não estão disponíveis.
Referências
BETTIOL, W.; MORANDI, M. A. B. Controle biológico de plantas no Brasil. 1. ed. Jaguariúna-SP: EmbrapaMeioAmbiente, 2009.
CHANDRASEKARAN, M.; BELACHEW, A. T.; YOON, E.; CHUN, S. Expression of β-1,3-glucanase (GLU) and phenylalanine ammonia-lyase (PAL) genes and their enzymes in tomato plants induced after treatment with Bacillus subtilis CBR05 against Xanthomonascampestrispv. vesicatoria. Journal of General Plant Pathology, Springer, v. 83, n. 1, p. 7-13, 2017.
EGAMBERDIEVA, D.; SHRIVASTAVA, S.; VARMA, A. Plant-Growth-Promoting Rhizobacteria (PGPR) and Medicinal Plants. Soil Biology, v 42, 2015.
EL HADRAMI, A. ADAM, L. R.; EL HADRAMI, I.; DAAYF, F. Chitosan in plant protection. Marine drugs, v. 8, n. 4, p. 968-87, 2010.
FAHEEM, M.; WASEEM, R.; WEI, Z. Evaluation of the biocontrol potential of Streptomyces goshikiensis YCXU against Fusariumoxysporum f. sp. niveum. BiologicalControl, v. 81, n. 1, p. 101-110, 2015.
FILGUEIRAS, L. M. B.; MENESES, C. H. S. G. Efeito das bactérias promotoras de crescimento de plantas na proteção contra o estresse hídrico. Journal of Biology & Pharmacy and Agricultural Management, v. 11, n. 1, p. 21-30, 2015.
HAN, J. H.; SHIN, H.; SHIN, J. H.; KIM, K. S. Antagonistic Activities of Bacillus spp. Strains Isolated from Tidal Flat Sediment Towards Anthracnose Pathogens Colletotrichumacutatum and C. gloeosporioides in South Korea. The plant pathology journal, v. 31, n. 2, p. 165-175, 2015.
HSU, S. C.; LOCKWOOD, J. L. Powdered chitin agar as a selective medium for enumeration of actinomycetes in water and soil. Applied and Environmental Microbiology, v. 29, n. 3, p. 422-426, 1975.
HUANG, X; ZHANG, N.; YONG, X.; YANG, X.; SHEN, Q. Biocontrol of Rhizoctoniasolani damping-off disease in cucumber with Bacillus pumilus SQR-N43.Microbiological Research, v. 167, n. 3, p. 135-143, 2012.
HUNGRIA, M.; ARAÚJO, R. M. Manual de Métodos Empregados em Estudos de Microbiologia Agrícola. Embrapa, 1994.
HUNGRIA, M.; CAMPO, R. J.; SOUZA, E. M.; PEDROSA, F. O. Inoculation with selected strains of Azospirillum brasilense and A. lipoferum improves yields of maize and wheat in Brazil. Plant and Soil, v. 331, n. 1-2, p. 413-425, 2010.
HAMID, R.; KHAN, M. A.; AHMAD, H.; ABDIN, M. Z.; MUSARRAT, J.; JAVED, S. Chitinases: An update. Journal of Pharmacy and Bioallied Sciences, v. 5, n. 1, p. 21-29, 2013.
JI, S. H.; PAUL, N. C.; DENG, J. X.; KIM, Y. S.; YUN, B.; YU, S. H. Biocontrol Activity of Bacillus amyloliquefaciens CNU114001 against Fungal Plant Diseases.Mycobiology, v. 41, n. 4, p. 234-242, 2013.
KEMPKA, A. P.; LIPKE, N. L.; PINHEIRO, L. F.; MENONCIN, S.; TREICHEL, H.; FREIRE, D. M.; DI LUCCIO, M.; OLIVEIRA, D. Response surface method to optimize the production and characterization of lipase from Penicilliumverrucosum in solid-state fermentation. Bioprocess and Biosystems Engineering, v. 31, n. 2, p. 119–125, 2008.
LEE, B.; FARAG, M. A.; PARK, H. B.; KLOEPPER, J. W.; LEE, S. H.; RYU, C. Induced resistance by a long-chain bacterial volatile: elicitation of plant systemic defense by a C13 volatile produced by Paenibacilluspolymyxa..PloS one, v. 7, n. 11, p. 1-11, 2012.
LI, C. H.; ZHAO, M. W.; TANG, C. M.; LI, S. P. Population dynamics and identification of endophytic bacteria antagonistic toward plant-pathogenic fungi in cotton root. Microbial ecology, v. 59, n. 2, p. 344-356, 2010.
LIU, W. Y.; WONG, C. F.; CHUNG, K. M. K.; JIANG, J. W.; LEUNG, F. C. C. Comparative genome analysis of Enterobacter cloacae. PLoS One, v. 8, n. 9, 2013.
MAKSIMOV, I. V.; ABIZGIL’DINA, R. R.; PUSENKOVA, L. I. Plant growth promoting rhizobacteria as alternative to chemical crop protectors from pathogens. AppliedBiochemistryandMicrobiology, v. 47, n. 4, p. 333-345, 2011.
MCTI, Ministério da Ciência, Tecnologia e Inovação. Estratégia nacional de ciência, tecnologia e inovação 2012 – 2015. Balanço das atividades estruturantes 2011, Brasilía: MCTI, 220 p., 2012.
MONTEIRO, L.; MARIANO, R. DE L. R.; MAIOR, A. M. S. AntagonismofBacillus spp. AgainstXanthomonascampestrispv. campestris. Brazilian Archives of Biology and Technology, v. 48, n. 1, p. 23–29, 2005.
MOURA, C. M.; MUSZINKI, P.; SCHMIDT, C.; ALMEIDA, J.; PINTO, L. A. A. Quitina e quitosana produzidas a partir de resíduos de camarão e siri: avaliação do processo em escala piloto. VETOR - Revista de Ciências Exatas e Engenharias, v. 16, n. 1, p. 37-45, 2006.
PEREIRA, E. L.; OLIVEIRA, A. F. A. A produção de antibióticos por processos fermentativos aeróbios. Revista da Universidade Vale do Rio Verde, Três Corações, v. 14, n. 2, p. 1058-1078, 2016
PÉREZ-PORTUONDO, I.; MERIÑO-REYES, L.; ÁBALOS-RODRÍGUEZ, A.; PÉREZ-SILVA, R. M. Características promotoras de crecimiento vegetal em rizobacteriasaisladas de suelos contaminados com compuestos fenólicos. Revista Cubana de Química, v. 29, n. 1, p. 73-88, 2017.
RAMPINO, A.; BORGOGNA, M.; BLASI, P.; BELLICH, B.; CESARO, A. Chitosannanoparticles: Preparation, sizeevolution and stability. International Journal of Pharmaceutics, v. 455, n. 1–2, p. 219– 228, 2013.
ROMICK, T. L.; FLEMING, H. P. Acetoin production as an indicator of growth and metabolic inhibition of Listeria monocytogenes. Journal of applied microbiology, v. 84, n. 1, p. 18-24, 1998.
RUDRAPPA, T.; BIEDRZYCHI, M. L.; KUNJETI, S. G.; DONOFRIO, N. M.; CZYMMEK, K. J.; PARÉ, P.; BAIS, H. P. The rhizobacterial elicitor acetoin induces systemic resistance inArabidopsis thaliana. CommunicativeandIntegrativeBiology, v. 3, n. 2, p. 130–138, 2010.
SÁNCHEZ, A. A. D.; LÓPEZ, M. C.; ARIAS, M. B.; CASTAÑOLL, I. E.; ABREU, M. P.; MADRUGAL, R. C. F.; HERNÁNDEZ, M. C. P.; ZUBIAR, Y. M. Bacterias patógenas de larvas de Bombyxmori L. en áreas de reproducciónen Cuba. Revista de Protección Vegetal, v. 29, n. 3, p. 216, 2015
SILVA, J. M.; RUARO, L.; MALAFAIA, G.; PAZ-LIMA, M. L. Seleção in vitro populações de microrganismos antagonistas e relacionamento contra a fusariose-do-feijoeiro (Fusariumoxysporumf.sp. phaseoli). Global Science and Technology, Rio Verde, v. 8, n. 1, p. 96-109, 2015.
SHALI, A.; GHASEMI, S.; AHMADIAN, G.; RANJBAR, G.; DEHESTANI, A.; KHALESI, N.; MOTALLEBI, E.; VAHED, M. Bacillus pumilus SG2 chitinases induced and regulated by chitin, show inhibitory activity against Fusariumgraminearum and Bipolarissorokiniana. Phytoparasitica, v. 38, n. 2, p. 141-147, 2010.
SPOLAOR, L. T.; GONÇALVES, L. S. A.; SANTOS, O. J. A. P. D.; OLIVEIRA, A. L. M. D.; SCAPIM, C. A.; BERTAGNA, F. A. B.; KUKI, M. C. Plant growth-promoting bacteria associated with nitrogen fertilization at topdressing in popcorn agronomic performance. Bragantia, v. 75, n. 1, p. 33-40, 2016.
STELATO, M. C. R. M. M. Microbiologia prática: Aplicações de aprendizagem de microbiologia básica, bactérias, fungos e vírus. 2. ed. ATHENEU, 2011.
SURYANTO, D.; IRAWATI, N.; MUNIR, E. Isolation and Characterization of Chitinolytic Bacteria and Their Potential to Inhibit Plant Pathogenic Fungi. Microbiology Indonesia, v. 5, n. 3, p. 8, 2012.
VELUSAMY, P.; KIM, K. Y. Chitinolytic activity of Enterobacter sp. KB3 antagonistic to Rhizoctoniasolani and its role in the degradation of living fungal hyphae. InternationalResearchJournalofMicrobiology, v. 2, n. 6, p. 206-214, 2011.
VIANA, F. M. P.; LIMA, J. R.; FEITOZA, E. M. B. A. C.; MEDEIROS, R. A. F. Produção de Sideróforos por Leveduras Antagônicas. Fortaleza: Embrapa, 2013.
WEN, C. M.; TSENG, C. S.; CHENG, C. Y.; LI, Y. K. Purification, characterization and cloning of a chitinase from Bacillus sp. NCTU2. Biotechnology and Applied Biochemistry, v. 35, n. 3, p. 213-219, 2002.
YASIR, M.; ASLAM, Z.; KIM, S. W.; LEE, S. W.; JEON, C. O.; CHUNG, Y. R. Bacterial community composition and chitinase gene diversity of vermicompost with antifungal activity. Bioresource Technology, v. 100, n. 19, p. 4396-4403, 2009.
ZALILA-KOLSI, I.; MAHMOUD, A. B.; ALI, H.; SELLAMI, S.; NASFI, Z.; TOUNSI, S.; JAMOUSSI, K. Antagonist effects of Bacillus spp. strains against Fusariumgraminearum for protection of durum wheat (Triticumturgidum L. subsp. durum). Microbiological Research, v. 192, p. 148-158, 2016.
ZHANG, X.; ZHOU, Y.; LI, Y.; FU, X.; WANG, Q. Screening and characterization of endophytic Bacillus for biocontrol of grapevine downy mildew. CropProtection, Elsevier, v. 96, n. 1, p. 173-179, 2017.
CHANDRASEKARAN, M.; BELACHEW, A. T.; YOON, E.; CHUN, S. Expression of β-1,3-glucanase (GLU) and phenylalanine ammonia-lyase (PAL) genes and their enzymes in tomato plants induced after treatment with Bacillus subtilis CBR05 against Xanthomonascampestrispv. vesicatoria. Journal of General Plant Pathology, Springer, v. 83, n. 1, p. 7-13, 2017.
EGAMBERDIEVA, D.; SHRIVASTAVA, S.; VARMA, A. Plant-Growth-Promoting Rhizobacteria (PGPR) and Medicinal Plants. Soil Biology, v 42, 2015.
EL HADRAMI, A. ADAM, L. R.; EL HADRAMI, I.; DAAYF, F. Chitosan in plant protection. Marine drugs, v. 8, n. 4, p. 968-87, 2010.
FAHEEM, M.; WASEEM, R.; WEI, Z. Evaluation of the biocontrol potential of Streptomyces goshikiensis YCXU against Fusariumoxysporum f. sp. niveum. BiologicalControl, v. 81, n. 1, p. 101-110, 2015.
FILGUEIRAS, L. M. B.; MENESES, C. H. S. G. Efeito das bactérias promotoras de crescimento de plantas na proteção contra o estresse hídrico. Journal of Biology & Pharmacy and Agricultural Management, v. 11, n. 1, p. 21-30, 2015.
HAN, J. H.; SHIN, H.; SHIN, J. H.; KIM, K. S. Antagonistic Activities of Bacillus spp. Strains Isolated from Tidal Flat Sediment Towards Anthracnose Pathogens Colletotrichumacutatum and C. gloeosporioides in South Korea. The plant pathology journal, v. 31, n. 2, p. 165-175, 2015.
HSU, S. C.; LOCKWOOD, J. L. Powdered chitin agar as a selective medium for enumeration of actinomycetes in water and soil. Applied and Environmental Microbiology, v. 29, n. 3, p. 422-426, 1975.
HUANG, X; ZHANG, N.; YONG, X.; YANG, X.; SHEN, Q. Biocontrol of Rhizoctoniasolani damping-off disease in cucumber with Bacillus pumilus SQR-N43.Microbiological Research, v. 167, n. 3, p. 135-143, 2012.
HUNGRIA, M.; ARAÚJO, R. M. Manual de Métodos Empregados em Estudos de Microbiologia Agrícola. Embrapa, 1994.
HUNGRIA, M.; CAMPO, R. J.; SOUZA, E. M.; PEDROSA, F. O. Inoculation with selected strains of Azospirillum brasilense and A. lipoferum improves yields of maize and wheat in Brazil. Plant and Soil, v. 331, n. 1-2, p. 413-425, 2010.
HAMID, R.; KHAN, M. A.; AHMAD, H.; ABDIN, M. Z.; MUSARRAT, J.; JAVED, S. Chitinases: An update. Journal of Pharmacy and Bioallied Sciences, v. 5, n. 1, p. 21-29, 2013.
JI, S. H.; PAUL, N. C.; DENG, J. X.; KIM, Y. S.; YUN, B.; YU, S. H. Biocontrol Activity of Bacillus amyloliquefaciens CNU114001 against Fungal Plant Diseases.Mycobiology, v. 41, n. 4, p. 234-242, 2013.
KEMPKA, A. P.; LIPKE, N. L.; PINHEIRO, L. F.; MENONCIN, S.; TREICHEL, H.; FREIRE, D. M.; DI LUCCIO, M.; OLIVEIRA, D. Response surface method to optimize the production and characterization of lipase from Penicilliumverrucosum in solid-state fermentation. Bioprocess and Biosystems Engineering, v. 31, n. 2, p. 119–125, 2008.
LEE, B.; FARAG, M. A.; PARK, H. B.; KLOEPPER, J. W.; LEE, S. H.; RYU, C. Induced resistance by a long-chain bacterial volatile: elicitation of plant systemic defense by a C13 volatile produced by Paenibacilluspolymyxa..PloS one, v. 7, n. 11, p. 1-11, 2012.
LI, C. H.; ZHAO, M. W.; TANG, C. M.; LI, S. P. Population dynamics and identification of endophytic bacteria antagonistic toward plant-pathogenic fungi in cotton root. Microbial ecology, v. 59, n. 2, p. 344-356, 2010.
LIU, W. Y.; WONG, C. F.; CHUNG, K. M. K.; JIANG, J. W.; LEUNG, F. C. C. Comparative genome analysis of Enterobacter cloacae. PLoS One, v. 8, n. 9, 2013.
MAKSIMOV, I. V.; ABIZGIL’DINA, R. R.; PUSENKOVA, L. I. Plant growth promoting rhizobacteria as alternative to chemical crop protectors from pathogens. AppliedBiochemistryandMicrobiology, v. 47, n. 4, p. 333-345, 2011.
MCTI, Ministério da Ciência, Tecnologia e Inovação. Estratégia nacional de ciência, tecnologia e inovação 2012 – 2015. Balanço das atividades estruturantes 2011, Brasilía: MCTI, 220 p., 2012.
MONTEIRO, L.; MARIANO, R. DE L. R.; MAIOR, A. M. S. AntagonismofBacillus spp. AgainstXanthomonascampestrispv. campestris. Brazilian Archives of Biology and Technology, v. 48, n. 1, p. 23–29, 2005.
MOURA, C. M.; MUSZINKI, P.; SCHMIDT, C.; ALMEIDA, J.; PINTO, L. A. A. Quitina e quitosana produzidas a partir de resíduos de camarão e siri: avaliação do processo em escala piloto. VETOR - Revista de Ciências Exatas e Engenharias, v. 16, n. 1, p. 37-45, 2006.
PEREIRA, E. L.; OLIVEIRA, A. F. A. A produção de antibióticos por processos fermentativos aeróbios. Revista da Universidade Vale do Rio Verde, Três Corações, v. 14, n. 2, p. 1058-1078, 2016
PÉREZ-PORTUONDO, I.; MERIÑO-REYES, L.; ÁBALOS-RODRÍGUEZ, A.; PÉREZ-SILVA, R. M. Características promotoras de crecimiento vegetal em rizobacteriasaisladas de suelos contaminados com compuestos fenólicos. Revista Cubana de Química, v. 29, n. 1, p. 73-88, 2017.
RAMPINO, A.; BORGOGNA, M.; BLASI, P.; BELLICH, B.; CESARO, A. Chitosannanoparticles: Preparation, sizeevolution and stability. International Journal of Pharmaceutics, v. 455, n. 1–2, p. 219– 228, 2013.
ROMICK, T. L.; FLEMING, H. P. Acetoin production as an indicator of growth and metabolic inhibition of Listeria monocytogenes. Journal of applied microbiology, v. 84, n. 1, p. 18-24, 1998.
RUDRAPPA, T.; BIEDRZYCHI, M. L.; KUNJETI, S. G.; DONOFRIO, N. M.; CZYMMEK, K. J.; PARÉ, P.; BAIS, H. P. The rhizobacterial elicitor acetoin induces systemic resistance inArabidopsis thaliana. CommunicativeandIntegrativeBiology, v. 3, n. 2, p. 130–138, 2010.
SÁNCHEZ, A. A. D.; LÓPEZ, M. C.; ARIAS, M. B.; CASTAÑOLL, I. E.; ABREU, M. P.; MADRUGAL, R. C. F.; HERNÁNDEZ, M. C. P.; ZUBIAR, Y. M. Bacterias patógenas de larvas de Bombyxmori L. en áreas de reproducciónen Cuba. Revista de Protección Vegetal, v. 29, n. 3, p. 216, 2015
SILVA, J. M.; RUARO, L.; MALAFAIA, G.; PAZ-LIMA, M. L. Seleção in vitro populações de microrganismos antagonistas e relacionamento contra a fusariose-do-feijoeiro (Fusariumoxysporumf.sp. phaseoli). Global Science and Technology, Rio Verde, v. 8, n. 1, p. 96-109, 2015.
SHALI, A.; GHASEMI, S.; AHMADIAN, G.; RANJBAR, G.; DEHESTANI, A.; KHALESI, N.; MOTALLEBI, E.; VAHED, M. Bacillus pumilus SG2 chitinases induced and regulated by chitin, show inhibitory activity against Fusariumgraminearum and Bipolarissorokiniana. Phytoparasitica, v. 38, n. 2, p. 141-147, 2010.
SPOLAOR, L. T.; GONÇALVES, L. S. A.; SANTOS, O. J. A. P. D.; OLIVEIRA, A. L. M. D.; SCAPIM, C. A.; BERTAGNA, F. A. B.; KUKI, M. C. Plant growth-promoting bacteria associated with nitrogen fertilization at topdressing in popcorn agronomic performance. Bragantia, v. 75, n. 1, p. 33-40, 2016.
STELATO, M. C. R. M. M. Microbiologia prática: Aplicações de aprendizagem de microbiologia básica, bactérias, fungos e vírus. 2. ed. ATHENEU, 2011.
SURYANTO, D.; IRAWATI, N.; MUNIR, E. Isolation and Characterization of Chitinolytic Bacteria and Their Potential to Inhibit Plant Pathogenic Fungi. Microbiology Indonesia, v. 5, n. 3, p. 8, 2012.
VELUSAMY, P.; KIM, K. Y. Chitinolytic activity of Enterobacter sp. KB3 antagonistic to Rhizoctoniasolani and its role in the degradation of living fungal hyphae. InternationalResearchJournalofMicrobiology, v. 2, n. 6, p. 206-214, 2011.
VIANA, F. M. P.; LIMA, J. R.; FEITOZA, E. M. B. A. C.; MEDEIROS, R. A. F. Produção de Sideróforos por Leveduras Antagônicas. Fortaleza: Embrapa, 2013.
WEN, C. M.; TSENG, C. S.; CHENG, C. Y.; LI, Y. K. Purification, characterization and cloning of a chitinase from Bacillus sp. NCTU2. Biotechnology and Applied Biochemistry, v. 35, n. 3, p. 213-219, 2002.
YASIR, M.; ASLAM, Z.; KIM, S. W.; LEE, S. W.; JEON, C. O.; CHUNG, Y. R. Bacterial community composition and chitinase gene diversity of vermicompost with antifungal activity. Bioresource Technology, v. 100, n. 19, p. 4396-4403, 2009.
ZALILA-KOLSI, I.; MAHMOUD, A. B.; ALI, H.; SELLAMI, S.; NASFI, Z.; TOUNSI, S.; JAMOUSSI, K. Antagonist effects of Bacillus spp. strains against Fusariumgraminearum for protection of durum wheat (Triticumturgidum L. subsp. durum). Microbiological Research, v. 192, p. 148-158, 2016.
ZHANG, X.; ZHOU, Y.; LI, Y.; FU, X.; WANG, Q. Screening and characterization of endophytic Bacillus for biocontrol of grapevine downy mildew. CropProtection, Elsevier, v. 96, n. 1, p. 173-179, 2017.
Downloads
Publicado
2021-01-13
Edição
Seção
Artigos
Licença
Copyright (c) 2021 Colloquium Agrariae. ISSN: 1809-8215
Este trabalho está licenciado sob uma licença Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
Como Citar
ACETOINE AND CHITINASE PRODUCTION BY NATIVE RIZOBACTERIA FROM WESTERN PARANÁ. (2021). Colloquium Agrariae. ISSN: 1809-8215, 16(6), 1-9. https://journal.unoeste.br/index.php/ca/article/view/3395