IN VITRO ASSESSMENT OF CYTOTOXICITY AND OXIDATIVE STRESS PARAMETERS OF NATURAL RUBBER LATEX MEMBRANES STABILIZED WITH DIFFERENT CONCENTRATIONS OF AMMONIA IN CHO-K1 CELLS

  • Caroline de Souza Araujo UNOESTE
  • Dalita Gomes Silva Morais Cavalcante Unesp
  • Nathália Oliveira Braga Unesp
  • Andressa Silva Gomes Unesp
  • Eidi Yoshihara APTA
  • Leandra Ernst Kerche UNOESTE
  • Aldo Eloizo Job Unesp
Palavras-chave: Natural rubber latex, membranes, ammonia, cytotoxicity, GSH.

Resumo

The purpose of this study was to assess in vitro cytotoxicity of natural rubber (NR) latex membranes stabilized with different concentrations of ammonia, as well as evaluate possible changes in oxidative stress parameters. To this end, CHO-k1 cells were exposed to NR membranes for 24 h. Cytotoxicity was assessed by the MTT assay and the oxidative stress parameters were established by glutathione (GSH) and malondialdehyde (MDA) levels. The results show that the ammonia present in the NR latex membranes can deplete cell viability and enhance GSH consumption in the cells. NR latex membranes stabilized with 2.00% of ammonia were cytotoxic to the cell lineage used in this study. From these results, it is possible to infer that the use of ammonia to stabilize NR latex membranes with biomedical purposes has to be done carefully, to avoid possible harmful effects to human health.

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Biografia do Autor

Leandra Ernst Kerche, UNOESTE
Graduada em Ciências Biológicas pela UEL (2005), Mestre em Biotecnologia pela UEL (2010) e Doutora em Biomateriais pela Unesp de Pres. Prudente, realiza pós-doutorado em Oncologia pelo AC Camargo Cancer Center. Orienta trabalhos com ênfase em Biomateriais, Mutagênese e Genética do Câncer.
Publicado
2020-01-17
Como Citar
Araujo, C. de S., Cavalcante, D. G. S. M., Braga, N. O., Gomes, A. S., Yoshihara, E., Kerche, L. E., & Job, A. E. (2020). IN VITRO ASSESSMENT OF CYTOTOXICITY AND OXIDATIVE STRESS PARAMETERS OF NATURAL RUBBER LATEX MEMBRANES STABILIZED WITH DIFFERENT CONCENTRATIONS OF AMMONIA IN CHO-K1 CELLS. Colloquium Vitae. ISSN: 1984-6436, 11(3), 32-40. Recuperado de http://journal.unoeste.br/index.php/cv/article/view/2446
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